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Enhancing cryopreservation of human induced pluripotent stem cells: bottom‐up versus conventional freezing geometry

2025-03-12Artigo científico Acesso restrito
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dc.contributor.authorMorais, Fernando Jorge Teodoro Duarte Garcia
dc.contributor.authorEl-Guendouz, Soukaina
dc.contributor.authorNeves, Rafaela
dc.contributor.authorDuarte, Andreia
dc.contributor.authorRodrigues, Miguel A.
dc.contributor.authorPinho Melo, Eduardo
dc.date.accessioned2025-03-21T13:16:44Z
dc.date.available2025-03-21T13:16:44Z
dc.date.issued2025-03-12
dc.description.abstractInduced pluripotent stem cells (iPSCs) hold large potential in regenerative medicine due to their pluripotency and unlimited self-renewal capacity without the ethical issues of embryonic stem cells. To provide quality-controlled iPSCs for clinical therapies, it is essential to develop safe cryopreservation protocols for long-term storage, preferably amenable to scale-up and automation. We have compared the impact of two different freezing geometries (bottom-up and conventional radial freezing) on the viability and differentiation potential of human iPSCs. Our results demonstrate that bottom-up freezing under optimized conditions significantly increases iPSC viability, up to 9% for cell membrane integrity and up to 21% for cell metabolic state, compared to conventional freezing. The improvement achieved for bottom-up versus conventional freezing was maintained after scale-up from cryogenic vials to 30 mL bags, highlighting its potential for clinical applications. These findings show that bottom-up freezing can offer a more controlled and scalable cryopreservation strategy for iPSCs, promoting their application in regenerative medicine.eng
dc.description.sponsorshipEMBRC.PT ALG-01-0145-FEDER-022121; LISBOA-01-0247-FEDER-047169
dc.identifier.doi10.1002/btpr.70019
dc.identifier.eissn1520-6033
dc.identifier.issn8756-7938
dc.identifier.urihttp://hdl.handle.net/10400.1/26940
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relation.ispartofBiotechnology Progress
dc.rights.uriN/A
dc.subjectBottom-up freezing
dc.subjectCryopreservation
dc.subjectInduced pluripotent stem cells
dc.subjectScale-up
dc.titleEnhancing cryopreservation of human induced pluripotent stem cells: bottom‐up versus conventional freezing geometryeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.titleBiotechnology Progress
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameMorais
person.familyNameEl-Guendouz
person.familyNamePinho Melo
person.givenNameFernando Jorge Teodoro Duarte Garcia
person.givenNameSoukaina
person.givenNameEduardo
person.identifierhttps://scholar.google.com/citations?user=CYBI8q8AAAAJ&hl=fr
person.identifier1443188
person.identifier.ciencia-id2517-06F1-0746
person.identifier.ciencia-id3E11-53A1-4D90
person.identifier.ciencia-id3C1C-C10C-1510
person.identifier.orcid0000-0001-5384-1469
person.identifier.orcid0000-0002-3341-9452
person.identifier.orcid0000-0002-0974-8977
person.identifier.scopus-author-id57190122389
person.identifier.scopus-author-id35566177900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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