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AVT is involved in the regulation of ion transport in the intestine of the sea bream (Sparus aurata)

2013Journal article Restricted
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dc.contributor.authorMartos-Sitcha, J. A.
dc.contributor.authorGregorio, Silvia
dc.contributor.authorCarvalho, Edison Samir Mascarelhas
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorPower, Deborah
dc.contributor.authorMancera, J. M.
dc.contributor.authorMartínez-Rodriguez, G.
dc.contributor.authorFuentes, J.
dc.date.accessioned2014-05-08T10:26:09Z
dc.date.available2014-05-08T10:26:09Z
dc.date.issued2013
dc.date.updated2014-05-08T09:32:02Z
dc.description.abstractThe intestine of marine fish plays a crucial role in ion homeostasis by selective processing of ingested fluid. Although arginine vasotocin (AVT) is suggested to play a role in ion regulation in fish, its action in the intestine has not been demonstrated. Thus, the present study investigated in vitro the putative role of AVT in intestinal ion transport in the sea bream (Sparus aurata). A cDNA encoding part of an AVT receptor was isolated and phylogenetic analysis revealed it clustered with the V1a2-type receptor clade. V1a2 transcripts were expressed throughout the gastrointestinal tract, from esophagus to rectum, and were most abundant in the rectum regardless of long-term exposure to external salinities of 12, 35 or 55 p.p.t. Basolateral addition of AVT (10 6 M) to the anterior intestine and rectum of sea bream adapted to 12, 35 or 55 p.p.t. mounted in Ussing chambers produced rapid salinity and region dependent responses in short circuit current (Isc), always in the absorptive direction. In addition, AVT stimulation of absorptive Isc conformed to a dose–response curve, with significant effects achieved at 10 8 M, which corresponds to physiological values of plasma AVT for this species. The effect of AVT on intestinal Isc was insensitive to the CFTR selective inhibitor NPPB (200 lM) applied apically, but was completely abolished in the presence of apical bumetanide (200 lM). We propose a role for AVT in the regulation of ion absorption in the intestine of the sea bream mediated by an absorptive bumetanide-sensitive mechanism, likely NKCC2.por
dc.identifier.citationMartos-Sitcha, Juan Antonio; Gregório, Silvia Filipa; Carvalho, Edison Samir M.; Canario, Adelino Vicente M.; Power, Deborah Mary; Mancera, Juan Miguel; Martínez-Rodríguez, Gonzalo; Fuentes, Juan. AVT is involved in the regulation of ion transport in the intestine of the sea bream (Sparus aurata), General and Comparative Endocrinology, 193, na, 221-228, 2013.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.ygcen.2013.07.017
dc.identifier.issn0016-6480
dc.identifier.otherAUT: ACA00258; DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/3808
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectOsmoregulationpor
dc.subjectArginine vasotocinpor
dc.subjectSea breampor
dc.subjectSalinitypor
dc.subjectWater absorptionpor
dc.titleAVT is involved in the regulation of ion transport in the intestine of the sea bream (Sparus aurata)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage228por
oaire.citation.startPage221por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume193por
person.familyNameGregorio
person.familyNameCanario
person.familyNamePower
person.familyNameFuentes
person.givenNameSilvia
person.givenNameAdelino
person.givenNameDeborah Mary
person.givenNameJuan
person.identifier1579837
person.identifier143624
person.identifier392998
person.identifier.ciencia-idBF14-020B-4C39
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id421B-E196-2C33
person.identifier.orcid0000-0002-6648-5122
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0003-0430-8734
person.identifier.ridG-1618-2018
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id7201832526
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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