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The effects of di-n-butyl phthalate and 4-tert-octylphenol in osteoclastic and osteoblastic activities in teleost fish scales

2015Conference object Open access
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dc.contributor.authorPinto, Patricia IS
dc.contributor.authorEstêvão, Dulce
dc.contributor.authorSantos, Soraia
dc.contributor.authorAndrade, André
dc.contributor.authorPower, Deborah
dc.date.accessioned2020-01-30T17:20:14Z
dc.date.available2020-01-30T17:20:14Z
dc.date.issued2015
dc.description.abstractDi-n-butyl phtalate (DBP) and 4-tert-octylphenol (OP) are environmental pollutants with estrogenic activity that have been shown to have endocrine disruptive actions in reproduction of several fish species. However, their impact in bone and scale metabolism, which are estrogen-responsive tissues, remains unknown. In this study, we evaluated the impact of these compounds on mineral metabolism in fish scales that, like bone, are a dynamic tissue maintained by continuous cycles of formation and resorption mediated, respectively, by osteoblasts (OSB) and osteoclasts (OSC). Using an in vitro bioassay, Atlantic sea bass (a marine species) and Mozambique tilapia (a freshwater species) scales were incubated with a range of concentrations of OP and DBP in culture media for a short (30 minutes) or long (24 hours) incubation time. Effects on the activity of tartrate resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP), markers for OSC and OSB activities, respectively, were assessed using a colorimetric enzymatic assay. DBP (10-6 M) affected TRAP activity in both species. While in sea bass, TRAP activity increased with DBP after 30 min incubation but was unaffected after 24 h, in tilapia no alterations were observed at the short term but a significant decrease was observed after 24 h incubation with this compound. None of the tested concentrations (10-10 to 10-6 M) affected ALP activity in both species. On the contrary, OP effects were only observed on the activity of ALP, which was significantly decreased after a 24 h incubation with 10-8 M of OP in the scales of both species. These results suggest that the exposure to these compounds may have disruptive effects on the metabolism of mineralized tissues in both marine and freshwater species. Future studies will investigate the mechanisms involved in these responses and the consequences for fish health.pt_PT
dc.description.sponsorshipFoundation for Science and Technology of Portugal (FCT), through projects PTDC/AAG-GLO/4003/2012 and PEst-C/MAR/LA0015/2011 and fellowship to PP (SFRH/BPD/84033/2012).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPinto, P., Estêvão, M. D., Santos, S., Andrade, A., Power D. M. (2015). The effects of di-n-butyl phthalate and 4-tert-octylphenol in osteoclastic and osteoblastic activities in teleost fish scales. 18th Pollutant Responses in Marine Organisms (PRIMO 18), Trondheim, 24-27/5/2015.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.1/13445
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationE2SCALE: Scaling-down estrogenic responses in fish
dc.titleThe effects of di-n-butyl phthalate and 4-tert-octylphenol in osteoclastic and osteoblastic activities in teleost fish scalespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleE2SCALE: Scaling-down estrogenic responses in fish
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAAG-GLO%2F4003%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F84033%2F2012/PT
oaire.citation.conferencePlaceTrondheimpt_PT
oaire.citation.title18th Pollutant Responses in Marine Organisms (PRIMO 18)pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
person.familyNamePinto
person.familyNameEstêvão
person.familyNameSantos
person.familyNameAlçada Baptista Rebelo de Andrade
person.familyNamePower
person.givenNamePatricia IS
person.givenNameMaria Dulce da Mota Antunes de Oliveira
person.givenNameSoraia
person.givenNameAndré
person.givenNameDeborah Mary
person.identifier643457
person.identifierI-4695-2014
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.ciencia-id881B-53E8-0DB1
person.identifier.ciencia-idEF18-F0AF-D388
person.identifier.ciencia-idF513-56ED-7088
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-7854-3898
person.identifier.orcid0000-0002-7151-8363
person.identifier.orcid0000-0001-5006-2021
person.identifier.orcid0000-0002-5900-8782
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-3817-2013
person.identifier.ridC-1328-2018
person.identifier.scopus-author-id10240774300
person.identifier.scopus-author-id8381590400
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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