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DNA damage and oxidative stress responses of mussels Mytilus galloprovincialis to paralytic shellfish toxins under warming and acidification conditions - Elucidation on the organ-specificity

2020-11Journal article Restricted access
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dc.contributor.authorBraga, Ana C.
dc.contributor.authorPereira, Vitoria
dc.contributor.authorMarcal, Raquel
dc.contributor.authorMarques, Ana
dc.contributor.authorGuilherme, Sofia
dc.contributor.authorReis Costa, Pedro
dc.contributor.authorPacheco, Mario
dc.date.accessioned2021-06-24T11:35:08Z
dc.date.available2021-06-24T11:35:08Z
dc.date.issued2020-11
dc.description.abstractCommonly affected by changes in climate and environmental conditions, coastal areas are very dynamic environments where shellfish play an important ecological role. In this study, the oxidative stress and genotoxic responses of mussels (Mytilus galloprovincialis) exposed to paralytic shellfish toxin (PST) - producing dinoflagellates Gymnodinium catenatum were evaluated under i) current conditions (CC: 19 degrees C; pH 8.0), warming (W: 24 degrees C; pH 8.0), acidification (A:19 degrees C; pH 7.6) and iv) combined effect of warming and acidification (WA: 24 degrees C; pH 7.6). Mussels were fed with G. catenatum for 5 days, and to a non-toxic diet during the following 10 days. A battery of oxidative stress biomarkers and comet assay was performed at the peak of toxin accumulation and at the end of the post-exposure phase. Under CC, gills and hepatopancreas displayed different responses/vulnerabilities and mechanisms to cope with PST. While gills presented a tendency for lipid peroxidation (LPO) and genetic damage (expressed by the Genetic Damage Indicator - GDI), hepatopancreas seems to better cope with the toxins, as no LPO was observed. However, the mechanisms involved in hepatopancreas protection were not enough to maintain DNA integrity. The absence of LPO, and the antioxidant system low responsiveness, suggests DNA damage was not oxidative. When exposed to toxic algae under W, toxin-modulated antioxidant responses were observed in both gills and hepatopancreas. Simultaneous exposure to the stressors highlighted gills susceptibility with a synergistic interaction increasing DNA damage. Exposure to toxic algae under A led to genotoxicity potentiation in both organs. The combined effect of WA did not cause relevant interactions in gills antioxidant responses, but stressors interactions impacted LPO and GDI. Antioxidant responses and LPO pointed out to be modulated by the environmental conditions in hepatopancreas, while GDI results support the dominance of toxin-triggered process. Overall, these results reveal that simultaneous exposure to warming, acidification and PSTs impairs mussel DNA integrity, compromising the genetic information due to the synergetic effects. Finally, this study highlights the increasing ecological risk of harmful algal blooms to Mytilus ganoprovincianis populations.
dc.description.sponsorshipCESAM [UIDP/50017/2020]
dc.description.sponsorshipFCT/MCTESPortuguese Foundation for Science and Technology [UIDP/50017/2020]
dc.description.sponsorshipPortuguese Foundation for Science and Technology (FCT)Portuguese Foundation for Science and Technology [UID/Multi/04326/2020]
dc.description.sponsorshipPortuguese Science and Technology Foundation (FCT)Portuguese Foundation for Science and Technology [PD/BD/113484/2015]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.aquatox.2020.105619
dc.identifier.issn0166-445X
dc.identifier.urihttp://hdl.handle.net/10400.1/16351
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.subjectHarmful algal blooms
dc.subjectClimate change
dc.subjectGenotoxicity
dc.subjectOxidative stress responses
dc.subjectSaxitoxins
dc.subjectGymnodinium catenatum
dc.subject.otherMarine & Freshwater Biology; Toxicology
dc.titleDNA damage and oxidative stress responses of mussels Mytilus galloprovincialis to paralytic shellfish toxins under warming and acidification conditions - Elucidation on the organ-specificity
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage105619
oaire.citation.titleAquatic Toxicology
oaire.citation.volume228
person.familyNameReis Costa
person.givenNamePedro
person.identifier600820
person.identifier.ciencia-idC911-9715-E547
person.identifier.orcid0000-0001-6083-470X
person.identifier.ridN-1908-2019
person.identifier.scopus-author-id7201895802
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication24ad7119-d61f-4bdb-a5d5-9bd6e2c37312
relation.isAuthorOfPublication.latestForDiscovery24ad7119-d61f-4bdb-a5d5-9bd6e2c37312

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