Comparing RAPD and AFLP (TM) analysis in discrimination and estimation of genetic similarities among apple (Malus domestica Borkh.) cultivars - RAPD and AFLP analysis of apples

Goulão, L.; Cabrita, L.; Oliveira, C.; Leitão, J. M.

http://hdl.handle.net/10400.1/6540

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Forty-one apple ( Malus x domestica Borkh.) cultivars were screened for RAPD (Random Amplified Polymorphic DNA) and AFLP (Amplified Fragment Length Polymorphism) markers. RAPD analysis was performed with 35 arbitrary 10-mer primers, selected from 60 primers tested (kits A, C and E, Operon Technologies, Inc.). Of a total of 362 bands observed, 208 (57.5%) were polymorphic. Three-hundred-and-eighty-one AFLP fragments were obtained with 8 primer combinations, of which 218 (57.2%) were polymorphic. Cultivars differentiated through mutation were included in this study and showed identical patterns when analysed with both RAPD and AFLP analysis. The estimated genetic relationships were correlated (r = 73.7%) between the analysis with the two different markers. UPGMA analysis was performed and dendrograms were constructed using either the data apart from each (RAPD and AFLP) method or combined in a single joint matrix. The relationships among the forty-one studied cultivars were basically consistent with the known lineage and geographic origins of the cultivars. The four Portuguese cultivars included in this study clustered together and diverged from the other cultivars. Apparently they constitute an independent genetic pool, which could be of interest for apple plant breeders.