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Proteomic changes in the extracellular environment of sea bass thymocytes exposed to 17α-ethinylestradiol in vitro

2021Journal article Restricted
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dc.contributor.authorMoreira, Catarina
dc.contributor.authorHétru, Julie
dc.contributor.authorPaiola, Matthieu
dc.contributor.authorDuflot, Aurélie
dc.contributor.authorChan, Philippe
dc.contributor.authorVaudry, David
dc.contributor.authorMonsinjon, Tiphaine
dc.contributor.authorKnigge, Thomas
dc.contributor.authorPinto, Patricia IS
dc.date.accessioned2021-11-10T20:26:06Z
dc.date.available2022-09-01T00:30:13Z
dc.date.issued2021
dc.description.abstractThe thymus is an important immune organ providing the necessary microenvironment for the development of a diverse, self-tolerant T cell repertoire, which is selected to allow for the recognition of foreign antigens while avoiding self-reactivity. Thymus function and activity are known to be regulated by sex steroid hormones, such as oestrogen, leading to sexual dimorphisms in immunocompetence between males and females. The oestrogenic modulation of the thymic function provides a potential target for environmental oestrogens, such as 17α-ethynylestradiol (EE2), to interfere with the cross-talk between the endocrine and the immune system. Oestrogen receptors have been identified on thymocytes and the thymic microenvironment, but it is unclear how oestrogens regulate thymic epithelial and T cell communication including paracrine signalling. Much less is known regarding intrathymic signalling in fish. Secretomics allows for the analysis of complex mixtures of immunomodulatory signalling factors secreted by T cells. Thus, in the present study, isolated thymocytes of the European sea bass, Dicentrarchus labrax, were exposed in vitro to 30 nM EE2 for 4 h and the T cell-secretome (i.e., extracellular proteome) was analysed by quantitative label-free mass-spectrometry. Progenesis revealed a total of 111 proteins differentially displayed between EE2-treated and control thymocytes at an α-level of 5% and a 1.3- fold change cut off (n = 5-6). The EE2-treatment significantly decreased the level of 90 proteins. Gene ontology revealed the proteasome to be the most impacted pathway. In contrast, the abundance of 21 proteins was significantly increased, with cathepsins showing the highest level of induction. However, no particular molecular pathway was significantly altered for these upregulated proteins. To the best of our knowledge, this work represents the first study of the secretome of the fish thymus exposed to the environmental oestrogen EE2, highlighting the impact on putative signalling pathways linked to immune surveillance, which may be of crucial importance for fish health and defence against pathogens.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.cbd.2021.100911pt_PT
dc.identifier.issn1744-117X
dc.identifier.urihttp://hdl.handle.net/10400.1/17314
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.relationNot Available
dc.subjectSecretomept_PT
dc.subjectImmune systempt_PT
dc.subjectThymuspt_PT
dc.subjectEndocrine disruptorpt_PT
dc.subjectDicentrarchus labraxpt_PT
dc.titleProteomic changes in the extracellular environment of sea bass thymocytes exposed to 17α-ethinylestradiol in vitropt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL 57%2F2016%2FCP1361%2FCT0015/PT
oaire.citation.startPage100911pt_PT
oaire.citation.titleComparative Biochemistry and Physiology Part D: Genomics and Proteomicspt_PT
oaire.citation.volume40pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamDL 57/2016
person.familyNamePinto
person.givenNamePatricia IS
person.identifier643457
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.orcid0000-0001-7854-3898
person.identifier.ridM-3817-2013
person.identifier.scopus-author-id10240774300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery64bc526e-5281-44e5-91eb-5743436109f9
relation.isProjectOfPublicationfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f
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