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Matrix Gla Protein expression pattern in the early avian embryo

2016Journal article Open access
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dc.contributor.authorCorreia, Elizabeth
dc.contributor.authorConceição, Natércia
dc.contributor.authorCancela, Leonor
dc.contributor.authorBelo, José A.
dc.date.accessioned2017-04-07T15:57:35Z
dc.date.available2017-04-07T15:57:35Z
dc.date.issued2016
dc.description.abstractMGP (Matrix Gla Protein) is an extracellular matrix vitamin K dependent protein previously identified as a physiological inhibitor of calcification and shown to be well conserved among vertebrates during evolution. MGP is involved in other mechanisms such as TGF-beta and BMP activity, and a proposed modulator of cell-matrix interactions. MGP is expressed early in vertebrate development although its role has not been clarified. Previous work in the chicken embryo found MGP localization predominantly in the aorta and aortic valve base, but no data is available earlier in development. Here we examined MGP expression pattern using whole-mount in situ hybridization and histological sectioning during the initial stages of chick development. MGP was first detected at HH10 in the head and in the forming dorsal aorta. At the moment of the onset of blood circulation, MGP was expressed additionally in the venous plexus which will remodel into the vitelline arteries. By E2.25, it is clear that the vitelline arteries are MGP positive. MGP expression progresses centrifugally throughout the area vasculosa of the yolk sac. Between stages HH17 and HH19 MGP is seen in the dorsal aorta, heart, notochord, nephric duct, roof plate, vitelline arteries and in the yolk sac, beneath main arterial branches and in the vicinity of several vessels and venules. MGP expression persists in these areas at least until E4.5. These data suggest that MGP expression could be associated with cell migration and differentiation and to the onset of angiogenesis in the developing chick embryo. This data has biomedical relevance by pointing to the potential use of chick embryo explants to study molecules involved in artery calcification.
dc.identifier.doi10.1387/ijdb.150365jb
dc.identifier.issn0214-6282
dc.identifier.otherAUT: LCA007; JBE01557;
dc.identifier.urihttp://hdl.handle.net/10400.1/9758
dc.language.isoeng
dc.peerreviewedyes
dc.publisherUniversity of the Basque Country Press
dc.relationStrategic Project - LA 23 - 2011-2012
dc.relationFunctional analysis of OPTN and SQSTM1 mutations in Paget´s disease of bone
dc.relation.isbasedonWOS:000375173000010
dc.subjectMGP
dc.subjectAngiogenesis
dc.subjectHeart development
dc.subjectBrain development
dc.subjectYolk sac
dc.titleMatrix Gla Protein expression pattern in the early avian embryo
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - LA 23 - 2011-2012
oaire.awardTitleFunctional analysis of OPTN and SQSTM1 mutations in Paget´s disease of bone
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0023%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F111898%2F2015/PT
oaire.citation.endPage76
oaire.citation.issue01-mar
oaire.citation.startPage71
oaire.citation.titleInternational Journal of Developmental Biology
oaire.citation.volume60
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream5876
person.familyNameCancela
person.familyNameBelo
person.givenNameM. Leonor
person.givenNameJosé A.
person.identifier.ciencia-idBF13-08E9-25E6
person.identifier.orcid0000-0003-3114-6662
person.identifier.orcid0000-0001-7384-0949
person.identifier.ridF-4444-2012
person.identifier.scopus-author-id6602141392
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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