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Comparative study on cellular and molecular responses in oyster sperm revealed different susceptibilities to cryopreservation

2019-01Journal article Restricted
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dc.contributor.authorF RIESCO, MARTA
dc.contributor.authorFélix, Francisca
dc.contributor.authorMatias, Domitilia
dc.contributor.authorJoaquim, Sandra
dc.contributor.authorSuquet, Marc
dc.contributor.authorCabrita, Elsa
dc.date.accessioned2020-07-24T10:53:36Z
dc.date.available2020-07-24T10:53:36Z
dc.date.issued2019-01
dc.description.abstractThe Portuguese (Crassostrea angulata) and Pacific oyster (Crassostrea gigas), both from the genus Crassostrea, are two important species for production and conservation. Although they have common characteristics, different susceptibilities to rearing conditions have been described in these species. Overall, in the case of C. angulata, only a few remaining populations are present in the south of Portugal and Spain. The preservation of genetic material from improved stocks or from the original population is crucial in oysters to prevent the potential impacts of epidemic diseases and natural disasters. Sperm cryopreservation in oysters has progressed in recent years. However many issues, such as protocol standardization, are still unsolved for the application of research results. In the present study a sperm cryopreservation protocol, previously published in C. angulata by our group, were analyzed in terms of cellular and molecular damage, in an effort to determine the most sensible parameters to standardize the cryopreservation protocols for both species. Different approaches in the analysis of sperm quality were performed for the first time in this genus to detect different susceptibilities between the two valuable species. Our results revealed that our previously published protocol containing 10% DMSO as cryoprotectant is more suitable comparing to 10% EG in both species. In addition, an integrative analysis was performed in both oyster species comparing all cellular parameters and C. gigas showed a higher susceptibility to cryopreservation using this optimized protocol. Moreover, higher susceptibility to transcript degradation was detected in C. gigas using this optimized cryopreservation protocol. This study highlights the importance of using different techniques and exhaustive analyses for selecting the most suitable cryopreservation protocol and its standardization, thus ensuring the total safety of the technique.
dc.description.sponsorshipPROMAR program [CRIOBIV-31-03-05-FEP-59]
dc.description.sponsorshipInterreg POCTEP [0139_VENUS_5_E]
dc.description.sponsorshipASSEMBLE + JRA2-H2020-INFRAIA [730984]
dc.description.sponsorshipInterreg Atlantic Area, European Regional Development Fund [EBB-EAPA_501/2016]
dc.description.sponsorshipCOST action (Food and Agriculture) AQUAGAMETE [FA1205]
dc.identifier.doi10.1016/j.aquaculture.2018.08.049
dc.identifier.issn0044-8486
dc.identifier.issn1873-5622
dc.identifier.urihttp://hdl.handle.net/10400.1/14512
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Science Bv
dc.relationAQUAculture infrastructures for EXCELlence in European fish research towards 2020
dc.subjectCrassostrea-gigas
dc.subjectPacific oyster
dc.subjectLipid-peroxidation
dc.subjectEmbryo development
dc.subjectHuman spermatozoa
dc.subjectGene-expression
dc.subjectMessenger-rnas
dc.subjectDna-damage
dc.subjectQuality
dc.subjectAngulata
dc.titleComparative study on cellular and molecular responses in oyster sperm revealed different susceptibilities to cryopreservation
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAQUAculture infrastructures for EXCELlence in European fish research towards 2020
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/652831/EU
oaire.citation.endPage229
oaire.citation.startPage223
oaire.citation.titleAquaculture
oaire.citation.volume498
oaire.fundingStreamH2020
person.familyNameF RIESCO
person.familyNameFélix de Azeredo Pinto e Melo
person.familyNameCabrita
person.givenNameMARTA
person.givenNameFrancisca
person.givenNameElsa
person.identifier.ciencia-idAC1C-FB89-E69C
person.identifier.orcid0000-0002-3655-6401
person.identifier.orcid0000-0003-3864-8841
person.identifier.ridO-9766-2014
person.identifier.ridE-8523-2012
person.identifier.scopus-author-id54796976600
person.identifier.scopus-author-id26645697000
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsrestrictedAccess
rcaap.typearticle
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relation.isAuthorOfPublication8a5633cd-dc8c-47ef-9d48-838e184d8225
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