Publication
Cytotoxic and hemolytic activities of extracts of the fish parasite dinoflagellate amyloodinium ocellatum
| dc.contributor.author | Moreira, Márcio | |
| dc.contributor.author | Soliño, Lucia | |
| dc.contributor.author | Marques, Cátia L. | |
| dc.contributor.author | Laizé, Vincent | |
| dc.contributor.author | Pousão-Ferreira, Pedro | |
| dc.contributor.author | Fidalgo E Costa, Pedro | |
| dc.contributor.author | Soares, Florbela | |
| dc.date.accessioned | 2022-07-26T12:52:45Z | |
| dc.date.available | 2022-07-26T12:52:45Z | |
| dc.date.issued | 2022-07-08 | |
| dc.date.updated | 2022-07-25T16:32:45Z | |
| dc.description.abstract | The dinoflagellate Amyloodinium ocellatum is the etiological agent of a parasitic disease named amyloodiniosis. Mortalities of diseased fish are usually attributed to anoxia, osmoregulatory impairment, or opportunistic bacterial infections. Nevertheless, the phylogenetic proximity of A. ocellatum to a group of toxin-producing dinoflagellates from Pfiesteria, Parvodinium and Paulsenella genera suggests that it may produce toxin-like compounds, adding a new dimension to the possible cause of mortalities in A. ocellatum outbreaks. To address this question, extracts prepared from different life stages of the parasite were tested in vitro for cytotoxic effects using two cell lines derived from branchial arches (ABSa15) and the caudal fin (CFSa1) of the gilthead seabream (Sparus aurata), and for hemolytic effects using erythrocytes purified from the blood of gilthead seabream juveniles. Cytotoxicity and a strong hemolytic effect, similar to those observed for Karlodinium toxins, were observed for the less polar extracts of the parasitic stage (trophont). A similar trend was observed for the less polar extracts of the infective stage (dinospores), although cell viability was only affected in the ABSa15 line. These results suggest that A. ocellatum produces tissue-specific toxic compounds that may have a role in the attachment of the dinospores’ and trophonts’ feeding process. | pt_PT |
| dc.description.sponsorship | MAR-02.05.01-FEAMP-0009; MAR-02.01.01-FEAMP-0175 | |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Toxins 14 (7): 467 (2022) | pt_PT |
| dc.identifier.doi | 10.3390/toxins14070467 | pt_PT |
| dc.identifier.eissn | 2072-6651 | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/18097 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | MDPI | pt_PT |
| dc.relation | Mechanisms of infection by the parasitic dinoflagellate Amyloodinium ocellatum, and physiological responses to infection in different marine fish species. | |
| dc.relation | The rise of toxic tropical and subtropical marine dinoflagellates Gambierdiscus spp: distribution, ciguatoxins trophic transfer and risk of ciguatera fish poisoning | |
| dc.relation | Algarve Centre for Marine Sciences | |
| dc.relation | Algarve Centre for Marine Sciences | |
| dc.relation | Centre for Marine and Environmental Research | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Fish pathology | pt_PT |
| dc.subject | Ectoparasite | pt_PT |
| dc.subject | Toxicological response | pt_PT |
| dc.subject | Hemolysis | pt_PT |
| dc.subject | Cell viability | pt_PT |
| dc.title | Cytotoxic and hemolytic activities of extracts of the fish parasite dinoflagellate amyloodinium ocellatum | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Mechanisms of infection by the parasitic dinoflagellate Amyloodinium ocellatum, and physiological responses to infection in different marine fish species. | |
| oaire.awardTitle | The rise of toxic tropical and subtropical marine dinoflagellates Gambierdiscus spp: distribution, ciguatoxins trophic transfer and risk of ciguatera fish poisoning | |
| oaire.awardTitle | Algarve Centre for Marine Sciences | |
| oaire.awardTitle | Algarve Centre for Marine Sciences | |
| oaire.awardTitle | Centre for Marine and Environmental Research | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F118601%2F2016/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/Concurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017/PTDC%2FCTA-AMB%2F30557%2F2017/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT | |
| oaire.citation.issue | 7 | pt_PT |
| oaire.citation.startPage | 467 | pt_PT |
| oaire.citation.title | Toxins | pt_PT |
| oaire.citation.volume | 14 | pt_PT |
| oaire.fundingStream | OE | |
| oaire.fundingStream | Concurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017 | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| person.familyName | Vicente Moreira | |
| person.familyName | Soliño | |
| person.familyName | Laizé | |
| person.familyName | Fidalgo e Costa | |
| person.givenName | Márcio Júlio | |
| person.givenName | Lucia | |
| person.givenName | Vincent | |
| person.givenName | Pedro | |
| person.identifier.ciencia-id | 6C13-12A0-2CFE | |
| person.identifier.ciencia-id | 2F12-68AC-8F6C | |
| person.identifier.orcid | 0000-0002-4932-4968 | |
| person.identifier.orcid | 0000-0001-8356-5688 | |
| person.identifier.orcid | 0000-0001-9565-9198 | |
| person.identifier.orcid | 0000-0003-3957-934X | |
| person.identifier.rid | B-4463-2008 | |
| person.identifier.scopus-author-id | 6602982778 | |
| person.identifier.scopus-author-id | 6507958996 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | 56b2d8ee-822a-4b2d-be90-1c0f2b346302 | |
| relation.isAuthorOfPublication | 27e1ebce-5931-49c1-9d91-53c69d8e255b | |
| relation.isAuthorOfPublication | b51cd3d5-7ee4-47d8-9bc4-b3e297aa222f | |
| relation.isAuthorOfPublication | 650cfc97-b17b-4404-84fb-1659b7584a0a | |
| relation.isAuthorOfPublication.latestForDiscovery | b51cd3d5-7ee4-47d8-9bc4-b3e297aa222f | |
| relation.isProjectOfPublication | d1be5763-6677-4001-98f2-77e530e6bcf3 | |
| relation.isProjectOfPublication | 59ac76d3-649a-40c2-9bfa-73dcf9b0e5bf | |
| relation.isProjectOfPublication | fafa76a6-2cd2-4a6d-a3c9-772f34d3b91f | |
| relation.isProjectOfPublication | 15f91d45-e070-47d8-b6b8-efd4de31d9a8 | |
| relation.isProjectOfPublication | 794d4c77-c731-471e-bc96-5a41dcd3d872 | |
| relation.isProjectOfPublication.latestForDiscovery | 794d4c77-c731-471e-bc96-5a41dcd3d872 |