A carregar...
Publicação
Cytotoxic and hemolytic activities of extracts of the fish parasite dinoflagellate amyloodinium ocellatum
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 3.13 MB | Adobe PDF |
Orientador(es)
Resumo(s)
The dinoflagellate Amyloodinium ocellatum is the etiological agent of a parasitic disease
named amyloodiniosis. Mortalities of diseased fish are usually attributed to anoxia, osmoregulatory
impairment, or opportunistic bacterial infections. Nevertheless, the phylogenetic proximity of
A. ocellatum to a group of toxin-producing dinoflagellates from Pfiesteria, Parvodinium and Paulsenella
genera suggests that it may produce toxin-like compounds, adding a new dimension to the possible
cause of mortalities in A. ocellatum outbreaks. To address this question, extracts prepared from
different life stages of the parasite were tested in vitro for cytotoxic effects using two cell lines derived
from branchial arches (ABSa15) and the caudal fin (CFSa1) of the gilthead seabream (Sparus aurata),
and for hemolytic effects using erythrocytes purified from the blood of gilthead seabream juveniles.
Cytotoxicity and a strong hemolytic effect, similar to those observed for Karlodinium toxins, were
observed for the less polar extracts of the parasitic stage (trophont). A similar trend was observed for
the less polar extracts of the infective stage (dinospores), although cell viability was only affected in
the ABSa15 line. These results suggest that A. ocellatum produces tissue-specific toxic compounds
that may have a role in the attachment of the dinospores’ and trophonts’ feeding process.
Descrição
Palavras-chave
Fish pathology Ectoparasite Toxicological response Hemolysis Cell viability
Contexto Educativo
Citação
Toxins 14 (7): 467 (2022)
Editora
MDPI