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Immunomodulatory effects of dietary methionine supplementation in rainbow trout (Oncorhynchus mykiss) juveniles: insights following vaccination and infection response against Yersinia ruckeri

2025-11-03Artigo científico Acesso aberto
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datacite.subject.sdg02:Erradicar a Fome
datacite.subject.sdg14:Proteger a Vida Marinha
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorCarvalho, Inês
dc.contributor.authorSchoninger, Felipe Bolgenhagen
dc.contributor.authorPereira da Cunha, André
dc.contributor.authorPeixoto, Diogo
dc.contributor.authorBrito, Francisca
dc.contributor.authorSimões, Luara
dc.contributor.authorVaz, Mariana
dc.contributor.authorStensballe, Allan
dc.contributor.authorFerreira, Inês
dc.contributor.authorSantos, Paulo
dc.contributor.authorTafalla, Carolina
dc.contributor.authorMachado, Marina
dc.contributor.authorCostas, Benjamín
dc.date.accessioned2026-05-12T09:46:48Z
dc.date.available2026-05-12T09:46:48Z
dc.date.issued2025-11-03
dc.description.abstractMethionine, an essential amino acid, participates in various pathways with implications for the immune system. Recent evidence suggests that it may support both innate and adaptive immune mechanisms. In the present study, it was hypothesized that dietary methionine supplementation prior to vaccination may be a promising strategy to improve vaccine efficacy. Hence, the current research aimed to evaluate the effects of dietary methionine supplementation on the immune status of rainbow trout (Oncorhynchus mykiss) juveniles, its role in modulating immune responses, as well as its potential synergistic effects with a commercial vaccine. To this end, fish were fed either a control diet (CTRL), meeting the methionine requirements of the species, or a methioninesupplemented diet (MET). After 4 weeks, half of the fish within each dietary group were either dip-vaccinated against Yersinia ruckeri (vaccinated) or left unvaccinated (naïve). Twenty-one days post-vaccination, during which fish continued on their respective dietary treatment, fish were intraperitoneally challenged with Y. ruckeri or injected with HBSS to serve as a control. Bacterial load in gills, posterior gut and spleen tissues, hematological parameters, differential cell counts, hepatic metabolites and antioxidant defenses, gene expression in the head-kidney and liver tissues, and plasma proteomic profiles were assessed following feeding trial and immunization period, and at early time points post-infection. Mortality was also monitored. Naïve fish exhibited a higher prevalence of Y. ruckeri, along with increased expression of pro-inflammatory and innate immune genes compared to their vaccinated counterparts. In contrast, vaccinated fish appeared to resolve the infection more rapidly, possibly through an early and heightened production of reactive oxygen species. In naïve fish, methionine supplementation appeared to impair antioxidant defenses and prolong immune activation, potentially contributing to the higher bacterial burden and reduced survival observed in this group. Differences between the two vaccinated groups were subtle, with no mortality recorded in either. However, proteomic analyses at 24 h post-infection revealed distinct responses, with MET-fed vaccinated fish exhibiting an increase in hemostasisrelated proteins, while CTRL-fed vaccinated fish showed a response more akin to pre-infection groups. Methionine supplementation in combination with vaccination appeared to promote slightly faster pathogen clearance.eng
dc.description.sponsorshipUI/BD/150900/2021
dc.identifier.doi10.3389/fimmu.2025.1706922
dc.identifier.issn1664-3224
dc.identifier.urihttp://hdl.handle.net/10400.1/28930
dc.language.isoeng
dc.peerreviewedyes
dc.publisherFrontiers Media SA
dc.relationThe modulatory role of tryptophan on the neuroendocrine and immune crosstalk
dc.relationMethionine and tryptophan as nutraceutical strategies to improve mucosal immunity and vaccine efficiency in fish
dc.relationBeyond methionine nutrition: new tools to improve fish robustness and disease resistance
dc.relationMethionine nutraceutical role in trout and turbot antiviral responses
dc.relationNutritional immunology as a tool to boost immunity and disease resistance in farmed fish
dc.relation.ispartofFrontiers in Immunology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectFunctional ingredients
dc.subjectAmino acids
dc.subjectFish immunity
dc.subjectEnteric redmouth disease
dc.subjectImmunization
dc.titleImmunomodulatory effects of dietary methionine supplementation in rainbow trout (Oncorhynchus mykiss) juveniles: insights following vaccination and infection response against Yersinia ruckerieng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUI/BD/150900/2021
oaire.awardNumberPTDC/CVT-CVT/7741/2020
oaire.awardNumber2021.04867.BD
oaire.awardNumber2022.03304.CEECIND/CP1728/CT0011
oaire.awardNumber2020.00290.CEECIND/CP1599/CT0001
oaire.awardTitleThe modulatory role of tryptophan on the neuroendocrine and immune crosstalk
oaire.awardTitleMethionine and tryptophan as nutraceutical strategies to improve mucosal immunity and vaccine efficiency in fish
oaire.awardTitleBeyond methionine nutrition: new tools to improve fish robustness and disease resistance
oaire.awardTitleMethionine nutraceutical role in trout and turbot antiviral responses
oaire.awardTitleNutritional immunology as a tool to boost immunity and disease resistance in farmed fish
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//UI%2FBD%2F150900%2F2021/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2020/PTDC%2FCVT-CVT%2F7741%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2021.04867.BD/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND5ed/2022.03304.CEECIND%2FCP1728%2FCT0011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 3ed/2020.00290.CEECIND%2FCP1599%2FCT0001/PT
oaire.citation.startPage1706922
oaire.citation.titleFrontiers in Immunology
oaire.citation.volume16
oaire.fundingStreamConcurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2020
oaire.fundingStreamCEEC IND5ed
oaire.fundingStreamCEEC IND 3ed
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNamePereira da Cunha
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