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Advisor(s)
Abstract(s)
As one of the most critical pathogens, Aeromonas hydrophila (AH) can cause motile aeromonad septicemia (MAS)
in freshwater fish. In recent decades, a myriad of studies had been done for bacterial infect fish. However, the
mechanism of bacterial infects fish especially freshwater fish was scanty. This study was conducted for investigating
the invasion pathway of A. hydrophila in vivo of black carp. We have performed A. hydrophila 4332
transformed with a plasmid encoding the green fluorescent protein (pGFPuv) (AH4332GFPuv) in black carp. The
AH4332GFPuv had similar growth properties and virulence as the wild-type strains under the simulated natural
condition. In this study, black carp were divided into five groups: IM (challenged via immersion), IBD (increased
stocking density), SAW (skin artificially wounded by scalpel), MR (mucus removed from the body surface), and
C0 (control group). The number of AH4332GFPuv in gill, liver, spleen, intestine, mid kidney, head kidney, muscle,
eye, brain, heart, and blood were examined after 72 h post-infection from all groups. Significantly high bacterial
numbers were observed in the gills and intestine. The number of bacteria was significantly higher in IBD group
than IM group. In conclusion, the gill, intestines, and injured skin are likely to be the primary infection routes.
Description
Keywords
Green fluorescent protein Infection dynamics Mylopharyngodon piceus Aeromonas hydrophila
Citation
Publisher
Elsevier