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Efeitos in vivo de oligómeros de vanadato em marcadores de stresse oxidativo
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Authors
Advisor(s)
Abstract(s)
Nowadays it is accepted that disturbances in transition metals
homeostasis are related to changes in the oxidative status, depleting
antioxidant substances and/or raising prooxidant ones or altering the
activities of key enzymes in the antioxidant defence. Vanadium is probably
involved in such effects. However when its toxicity is studied it is normally
misregarded the formation of different vanadate oligomers with different
structures, chemical properties and biochemical effects. Liver is one of the
organs affected by transition-metals toxicity due to its accumulation.
This work’s goal was to seek evidences that show different effects
promoted by different vanadate oligomers. We have monitored several
markers of oxidative stress by using fluorometric and spectroscopic
techniques, and we analysed the in vivo effect of an acute exposure (12, 24
hours and 7 days), to a sub-lethal concentration (5 mM; 1 mg/kg) of two
vanadium solutions (metavanadate and decavanadate), on hepatic tissue
of Halobatrachus didactylus (toadfish).
We observed that decavanadate promote different effects than
metavanadate in several parameters, namely catalase activity, glutathione
content, lipid peroxidation, mitochondrial superoxide anion production and
vanadium accumulation. The results show that under defined experimental
conditions, both solutions seem to equally depress ROS production as well as
total intracellular reducing power.
Description
Relatório de estágio de licenciatura, Bioquímica, Faculdade de Ciências e Tecnologia. Universidade do Algarve, 2003
Keywords
Vanadato Stress oxidativo