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Magnetic nanoparticles for biosensing and immunoprecipitation

2019-09-16Master thesis Open access
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datacite.subject.fosEngenharia e Tecnologia::Outras Engenharias e Tecnologiaspt_PT
dc.contributor.advisorCosta, Ana M. Rosa da
dc.contributor.advisorSantander, José María Palacios
dc.contributor.authorBeltran, Christian Hazael Perez
dc.date.accessioned2020-05-25T17:07:25Z
dc.date.available2020-05-25T17:07:25Z
dc.date.issued2019-09-16
dc.description.abstractOur world is rapidly changing and its future is on our hands. Great effort is being done against overexploitation of natural resources, uncontrolled hunting and pollution. A great concerning fact is due to pollution which is causing a continuous greenhouse effect and new cancer cases every single day. Nowadays, it is possible to improve the detection of lethal elements in the environment, to fight against cancer in a smarter manner, with less pain and with more efficiency but, more important, to use the same low-cost, fast and environmentally friendly tool for these purposes and more. This reality is thanks to previous works and findings regarding the Magnetic Nanoparticles (MNPs), which are employable in a wide variety of applications such as magnetic recording media, resonance imaging, heavy metals ions removal and biomedicine (specifically in the hyperthermic treatment of malignant cells, site-specific drug delivery and separation of proteins and cell population). MNPs have special properties such as superparamagnetic, high field irreversibility, high saturation field, extra anisotropy contributions or shifted loops after field cooling, biocompatibility, long durability, low toxicity and cost. In this context, this project intends 1) to develop through a novel synthesis method, a biosensor capable to detect mercury in water by irreversible inhibition of the enzyme Horseradish Peroxidase attached onto the surface of different coated MNPs being able to approximate its detections to those limits stablished by the Environmental Protecting Agency of the United States of America; and 2) to use these high valuable nanoparticles as an immunoprecipitation vehicle through the attachment of a polyclonal antibody onto the surface of functionalized MNPs, selective against a suppressor protein. MNPs of about 10 nm were obtained within one minute via co-precipitation method enhanced by high power ultrasound. Experimental design has been used in order to optimize the preparation process from hours to just one minute. The composition, structure, size and morphology analyses of these MNPs have been carried out through X-ray diffraction, Fourier transform infrared spectroscopy, thermogravimetric analysis and scanning electron microscopy showing the correct achievement of the MNPs. Moreover, different coating agents have been tested in order to functionalize MNPs surface with the aim of attaching later biomolecules, such as enzymes and antibodies.pt_PT
dc.identifier.tid202468330pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.1/13939
dc.language.isoengpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMagnetic nanoparticles (MNPs)pt_PT
dc.subjectEnvironmental controlpt_PT
dc.subjectBiosensorpt_PT
dc.subjectIrreversible inhibitionpt_PT
dc.subjectBiomedicinept_PT
dc.subjectImmunoprecipitationpt_PT
dc.subjectSonocatalysispt_PT
dc.subjectGreen analytical chemistrypt_PT
dc.subjectDesign of experimentspt_PT
dc.titleMagnetic nanoparticles for biosensing and immunoprecipitationpt_PT
dc.typemaster thesis
dspace.entity.typePublication
rcaap.rightsopenAccesspt_PT
rcaap.typemasterThesispt_PT
thesis.degree.grantorUniversidade do Algarve. Faculdade de Ciências e Tecnologia
thesis.degree.levelMestre
thesis.degree.nameMestrado em Qualidade em Análises - Erasmus Munduspt_PT

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