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Changes in protein expression in mussels Mytilus galloprovincialis dietarily exposed to PVP/PEI coated silver nanoparticles at different seasons

2019Journal article Open access
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dc.contributor.authorDuroudier, Nerea
dc.contributor.authorCardoso, Cátia
dc.contributor.authorMehennaoui, Kahina
dc.contributor.authorMikolaczyk, Mathilde
dc.contributor.authorSchäfer, Jörg
dc.contributor.authorGutleb, Arno C.
dc.contributor.authorGiamberini, Laure
dc.contributor.authorBebianno, Maria João
dc.contributor.authorBilbao, Eider
dc.contributor.authorCajaraville, Miren P.
dc.date.accessioned2019-02-26T13:04:53Z
dc.date.available2021-02-01T01:30:13Z
dc.date.issued2019
dc.description.abstractPotential toxic effects of Ag NPs ingested through the food web and depending on the season have not been addressed in marine bivalves. This work aimed to assess differences in protein expression in the digestive gland of female mussels after dietary exposure to Ag NPs in autumn and spring. Mussels were fed daily with microalgae previously exposed for 24 hours to 10 µg/L of PVP/PEI coated 5 nm Ag NPs. After 21 days, mussels significantly accumulated Ag in both seasons and Ag NPs were found within digestive gland cells and gills. Two-dimensional electrophoresis distinguished 104 differentially expressed protein spots in autumn and 142 in spring. Among them, chitinase like protein-3, partial and glyceraldehyde-3-phosphate dehydrogenase, that are involved in amino sugar and nucleotide sugar metabolism, carbon metabolism, glycolysis/gluconeogenesis and the biosynthesis of amino acids KEGG pathways, were overexpressed in autumn but underexpressed in spring. In autumn, pyruvate metabolism, citrate cycle, cysteine and methionine metabolism and glyoxylate and dicarboxylate metabolism were altered, while in spring, proteins related to the formation of phagosomes and hydrogen peroxide metabolism were differentially expressed. Overall, protein expression signatures depended on season and Ag NPs exposure, suggesting that season significantly influences responses of mussels to NP exposure.pt_PT
dc.description.sponsorshipThis work has been funded by the Spanish Ministry of Economy and Competitiveness (NanoSilverOmics project MAT2012-39372), Basque Government (SAIOTEK project S-PE13UN142 and Consolidated Research Group GIC IT810-13) and the University of the Basque Country UPV/EHU (UFI 11/37 and PhD fellowship to N.D.). This study had also the support of Fundação para a Ciência e Tecnologia (FCT) from Portugal through the Strategic Project UID/MAH00350/2013 granted to CIMA. The contribution of K. Mehennaoui was possible within the project NanoGAM (AFR-PhD-9229040) and M. Mikolaczyk was supported by a PhD fellowship from the French Ministry of Higher Education and Research.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.doi10.1016/j.aquatox.2019.02.010pt_PT
dc.identifier.issn0166-445X
dc.identifier.urihttp://hdl.handle.net/10400.1/12375
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0166445X18310269?via%3Dihubpt_PT
dc.subjectSilver nanoparticlespt_PT
dc.subjectDietary exposurept_PT
dc.subjectMussels Mytilus galloprovincialispt_PT
dc.subjectSeasonspt_PT
dc.subjectProteomic analysispt_PT
dc.titleChanges in protein expression in mussels Mytilus galloprovincialis dietarily exposed to PVP/PEI coated silver nanoparticles at different seasonspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleAquatic Toxicologypt_PT
person.familyNameCardoso
person.familyNameBebianno
person.givenNameCátia
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-3157-9898
person.identifier.orcid0000-0003-1492-8566
person.identifier.ridI-9235-2014
person.identifier.scopus-author-id7004152715
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2cf7d59f-e432-46f3-a411-7085113ea2a7
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32

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