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Thermal conditions during larval pelagic phase influence subsequent somatic growth of Senegalese sole by modulating gene expression and muscle growth dynamics

2013Journal article Restricted access
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dc.contributor.authorCampos, C.
dc.contributor.authorFernandes, J. M. O.
dc.contributor.authorConceição, L. E. C.
dc.contributor.authorEngrola, S.
dc.contributor.authorSousa, V.
dc.contributor.authorValente, L. M. P.
dc.date.accessioned2014-07-03T14:45:25Z
dc.date.available2014-07-03T14:45:25Z
dc.date.issued2013
dc.date.updated2014-07-03T11:00:30Z
dc.description.abstractIn the present study, Senegalese sole eggs incubated at 20 °C were reared at three different temperatures (15 °C, 18 °C or 21 °C) during the pelagic phase, and then transferred to a common temperature (20 °C) from benthic stage until 100 days post-hatch (dph). Somatic growth, fast muscle cellularity and expression of 15 growth-related genes were compared at the same developmental stage for each temperature (pre-metamorphic larvae, metamorphic larvae, post-metamorphic larvae) and at 83 dph and 100 dph early juveniles. During pre-metamorphosis and metamorphosis, larvae from 21 °C weighed significantly more than those reared at 18 °C or 15 °C (P < 0.001). Relative growth rate (RGR) of pelagic larvae and survival of newly-settled larvae were also higher at 21 °C (P < 0.05). Furthermore, an increase in muscle growth towards the highest temperatures was observed concomitantly with an increase in gene expression, namely myogenic regulatory factors (MRFs), myosins, igf-I and fgf6. After transfer to a common temperature (20 °C), the 15 °C group initiated a process of compensatory growth, inverting relative growth rate values that became the highest, particularly between 83 dph and 100 dph (P < 0.05). This increased growth effort in the 15 °C group was accompanied by an up-regulation of gene expression in fast muscle, particularly in the 83 dph juveniles. Mrf4 and myHC were up-regulated at 15 °C and positive correlations with growth were also found for myog or pax7 (P < 0.05). In contrast, mstn1 was down-regulated at 15 °C (P < 0.05), suggesting a boost in muscle growth. By 100 dph, the 15 °C group had already reached the same weight as the 18 °C and the same length as the 21 °C ones. At this age, the 18 °C juveniles had the largest mean muscle fibre diameter (P < 0.001), and thus fibre hypertrophy seems to be a major growth mechanism relatively to the other groups. Our work shows that temperature during pelagic phase greatly influences the growth trajectory of Senegalese sole juveniles.por
dc.identifier.citationCampos, C.; Fernandes, J.M.O.; Conceição, L.E.C.; Engrola, S.; Sousa, V.; Valente, L.M.P.Thermal conditions during larval pelagic phase influence subsequent somatic growth of Senegalese sole by modulating gene expression and muscle growth dynamics, Aquaculture, 414, s/n, 46-55, 2013.por
dc.identifier.doihttp://dx.doi.org/ 10.1016/j.aquaculture.2013.07.039
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10400.1/4644
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0044848613003773por
dc.subjectMyogenesispor
dc.subjectMuscle cellularitypor
dc.subjectSolea senegalensispor
dc.subjectThermoregulation of myogenesispor
dc.subjectGrowth-related genespor
dc.titleThermal conditions during larval pelagic phase influence subsequent somatic growth of Senegalese sole by modulating gene expression and muscle growth dynamicspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage55por
oaire.citation.startPage46por
oaire.citation.titleAquaculturepor
oaire.citation.volume414-415por
person.familyNameEngrola
person.givenNameSofia
person.identifier237846
person.identifier.ciencia-idA610-29E7-9B83
person.identifier.orcid0000-0002-5244-5541
person.identifier.ridA-2485-2012
person.identifier.scopus-author-id8669620600
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication51be5a20-df9c-4a90-85eb-51d779642caa
relation.isAuthorOfPublication.latestForDiscovery51be5a20-df9c-4a90-85eb-51d779642caa

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