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Effect of creatine and EDTA supplemented diets on European seabass (Dicentrarchus labrax) allergenicity, fish muscle quality and omics fingerprint

2022Journal article Restricted access
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dc.contributor.authorSchrama, Denise
dc.contributor.authorRaposo de Magalhães, Cláudia
dc.contributor.authorCerqueira, Marco
dc.contributor.authorCarrilho, Raquel
dc.contributor.authorFarinha, Ana Paula
dc.contributor.authorCosta, Ana Rosa da
dc.contributor.authorGonçalves, Amparo
dc.contributor.authorKuehn, Annette
dc.contributor.authorRevets, Dominique
dc.contributor.authorPlanchon, Sébastien
dc.contributor.authorEngrola, Sofia
dc.contributor.authorRodrigues, Pedro
dc.date.accessioned2022-02-22T14:27:24Z
dc.date.available2022-02-22T14:27:24Z
dc.date.issued2022
dc.description.abstractThe relatively easy access to fish worldwide, alongside the increase of aquaculture production contributes to increased fish consumption which result in higher prevalence of respective allergies. Allergies to fish constitute a significant concern worldwide. beta-parvalbumin is the main elicitor for IgE-mediated reactions. Creatine, involved in the muscle energy metabolism, and ethylenediamine tetraacetic acid (EDTA), a calcium chelator, are potential molecules to modulate parvalbumin. The purpose of this study was to test creatine (2, 5 and 8%) and EDTA (1.5, 3 and 4.5%) supplementation in fish diets to modulate beta-parvalbumin expression and structure and its allergenicity in farmed European seabass (Dicentrarchus labrax) while assessing its effects on the end-product quality. Fish welfare and muscle quality parameters were evaluated by plasma metabolites, rigor mortis, muscle pH and sensory and texture analysis. Proteomics was used to assess alterations in muscle proteome profile and metabolic fingerprinting by Fourier transform infrared spectroscopy was used to assess the liver metabolic profile. In addition, IgE-reactivity to parvalbumin was analysed using fish allergic patient sera. Metabolic fingerprinting of liver tissue revealed no major alterations in infrared spectra with creatine supplementation, while with EDTA, only absorption bands characteristic of lipids were altered. Comparative proteomics showed up regulation of (tropo) myosin and phosphoglycerate mutase 2 with Creatine supplementation. In the case of EDTA proteomics showed up regulation of proteins involved in cellular and ion homeostasis. Allergenicity seems not to be modulated with creatine or EDTA supplementation as no decreased expression levels were found and IgE-binding reactivity showed no quantitative differences.pt_PT
dc.description.sponsorship16-02-01-FMP-0014
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.cbd.2021.100941pt_PT
dc.identifier.issn1744-117X
dc.identifier.urihttp://hdl.handle.net/10400.1/17588
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCharacterization and modulation of fish allergenicity towards the production of a low allergen farmed fish
dc.relationEstablishment of farmed fish welfare biomarkers; a multi-omics approach
dc.relationAlgarve Centre for Marine Sciences
dc.subjectAllergenicitypt_PT
dc.subjectCreatinept_PT
dc.subjectEDTApt_PT
dc.subjectEuropean seabasspt_PT
dc.subjectMuscle qualitypt_PT
dc.titleEffect of creatine and EDTA supplemented diets on European seabass (Dicentrarchus labrax) allergenicity, fish muscle quality and omics fingerprintpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCharacterization and modulation of fish allergenicity towards the production of a low allergen farmed fish
oaire.awardTitleEstablishment of farmed fish welfare biomarkers; a multi-omics approach
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F136319%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F138884%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.citation.startPage100941pt_PT
oaire.citation.titleComparative Biochemistry and Physiology Part D: Genomics and Proteomicspt_PT
oaire.citation.volume41pt_PT
oaire.fundingStreamOE
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSchrama
person.familyNameRaposo de Magalhães
person.familyNameCerqueira
person.familyNameVaz Carrilho
person.familyNameMARTINS FARINHA RESENDE
person.familyNameRosa da Costa
person.familyNameRodrigues
person.givenNameDenise
person.givenNameCláudia
person.givenNameMarco
person.givenNameRaquel
person.givenNameANA PAULA
person.givenNameAna M
person.givenNamePedro
person.identifier159154
person.identifierG-5692-2017
person.identifier305029
person.identifier.ciencia-id501C-AC69-68C0
person.identifier.ciencia-idEB1C-0E40-F003
person.identifier.ciencia-id4F1E-16FC-00D8
person.identifier.ciencia-idF41E-AC88-8BCD
person.identifier.ciencia-id4312-4B8F-3079
person.identifier.ciencia-idA418-A85E-5DB1
person.identifier.orcid0000-0003-0274-5957
person.identifier.orcid0000-0001-7751-2870
person.identifier.orcid0000-0001-7237-5053
person.identifier.orcid0000-0003-1455-5958
person.identifier.orcid0000-0003-0225-9537
person.identifier.orcid0000-0002-9668-1204
person.identifier.ridH-6511-2016
person.identifier.ridE-2165-2012
person.identifier.ridM-3406-2013
person.identifier.scopus-author-id55755583400
person.identifier.scopus-author-id54386717500
person.identifier.scopus-author-id53986075100
person.identifier.scopus-author-id55107531000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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