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One-minute and green synthesis of magnetic iron oxide nanoparticles assisted by design of experiments and high energy ultrasound: Application to biosensing and immunoprecipitation

2021-04Journal article Restricted
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dc.contributor.authorPérez-Beltrán, Christian Hazael
dc.contributor.authorJose Garcia-Guzman, Juan
dc.contributor.authorFerreira, Bibiana
dc.contributor.authorEstevez-Hernandez, Osvaldo
dc.contributor.authorLopez-Iglesias, David
dc.contributor.authorCubillana-Aguilera, Laura
dc.contributor.authorLink, Wolfgang
dc.contributor.authorStanica, N.
dc.contributor.authorRosa Da Costa, Ana
dc.contributor.authorMaria Palacios-Santander, Jose
dc.date.accessioned2021-09-08T10:58:00Z
dc.date.available2021-09-08T10:58:00Z
dc.date.issued2021-04
dc.description.abstractThe present study is focused on the ultrafast and green synthesis, via the co-precipitation method, of magnetic nanoparticles (MNPs) based on iron oxides using design of experiments (DOE) and high energy sonochemical approach, considering two main factors: amplitude (energy) of the ultrasound probe and sonication time. The combination of these techniques allowed the development of a novel one-minute green synthesis, which drastically reduced the amount of consumed energy, solvents, reagents, time and produced residues. This green sonochemical synthesis permitted to obtain mean particle sizes of 11 ? 2 nm under the optimized conditions of amplitude = 40% (2826 J) and time = 1 min. Their composition, structure, size, morphology and magnetic properties were assessed through X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), scanning and transmission electron microscopy (SEM & TEM), and vibrating sample magnetometry (VSM). The characterization results indicate the proper formation of MNPs, and the correct functionalization of MNPs with different coating agents. The functionalized MNPs were used as: i) biosensor, which could detect mercury in water in the range of 0.030?0.060 ppm, and ii) support onto which polyclonal antibodies were anchored and successfully bound to an osteosarcoma cell line expressing the target protein (TRIB2-GFP), as part of an immunoprecipitation assay.
dc.description.sponsorshipUniversidad Autonoma de Sinaloa (Mexico); Erasmus Mundus Master in Quality in Analytical Laboratories (EMQAL); Junta de Andalucia; Institute of Research on Electron Microscopy and Materials (IMEYMAT -BIOSENSEP project); Institute of Research on Electron Microscopy and Materials (APPLIED-SENS project); Programa de fomento e impulso de la investigacion y de la transferencia de la Universidad de Cadiz 2018-2019 [PR2018-070, PR2020-013/SMAGAC]; Asociacion Universitaria Iberoamericana de Posgrado (AUIP); Fundacao para a Ciencia e a Tecnologia (FCT)Portuguese Foundation for Science and TechnologyEuropean Commission [UID/BIM/04773/2013, UID/QUI/04023/2019]; Spanish Ministry of Science, Innovation and Universities [RTI2018-094629-B-I00]; Marie Curie Individual Fellowship project TRIBBLES [748585]; LPCC-NRS/Terry Fox grant (2016/2017); LPCC-NRS/Terry Fox grant (2017/2018); [FCT-SFRH/BPD/100434/2014]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.msec.2021.112023
dc.identifier.issn0928-4931
dc.identifier.urihttp://hdl.handle.net/10400.1/16999
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationCentro de Investigação em Química do Algarve
dc.relationCentre for Biomedical Research
dc.relationCharacterizing the clinical relevance and the mechanism underlying TRIB2-mediated drug resistance to MEK inhibitiors in the context of melanoma
dc.relationCharacterizing the clinical relevance of TRIB2 mediated resistance to PI3K pathway inhibition in colon and breast cancer
dc.subjectMagnetic nanoparticles
dc.subject1 min-synthesis
dc.subjectDesign of experiments
dc.subjectHigh-energy ultrasound
dc.subjectIron oxides
dc.subjectBiosensing
dc.subjectImmunoprecipitation
dc.subject.otherMaterials Science
dc.titleOne-minute and green synthesis of magnetic iron oxide nanoparticles assisted by design of experiments and high energy ultrasound: Application to biosensing and immunoprecipitation
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentro de Investigação em Química do Algarve
oaire.awardTitleCentre for Biomedical Research
oaire.awardTitleCharacterizing the clinical relevance and the mechanism underlying TRIB2-mediated drug resistance to MEK inhibitiors in the context of melanoma
oaire.awardTitleCharacterizing the clinical relevance of TRIB2 mediated resistance to PI3K pathway inhibition in colon and breast cancer
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F04773%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FQUI%2F04023%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIM%2F04773%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/748585/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBPD%2F100434%2F2014/PT
oaire.citation.startPage112023
oaire.citation.titleMaterials Science & Engineering C-Materials For Biological Applications
oaire.citation.volume123
oaire.fundingStream5876
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamH2020
oaire.fundingStreamOE
person.familyNamePérez-Beltrán
person.familyNameferreira
person.familyNameRosa da Costa
person.givenNameChristian Hazael
person.givenNameBibiana
person.givenNameAna M
person.identifier305029
person.identifier.ciencia-idA311-E925-09C5
person.identifier.ciencia-idA418-A85E-5DB1
person.identifier.orcid0000-0003-0957-2542
person.identifier.orcid0000-0003-4772-9395
person.identifier.orcid0000-0003-0225-9537
person.identifier.ridE-2165-2012
person.identifier.scopus-author-id57222383439
person.identifier.scopus-author-id53986075100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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