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Stress responses in Crassostrea gasar exposed to combined effects of acute pH changes and phenanthrene

2019Journal article Restricted access
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dc.contributor.authorLima, Daína
dc.contributor.authorMattos, Jacó J.
dc.contributor.authorPiazza, Rômi S.
dc.contributor.authorRighetti, Bárbara Pacheco Harrison
dc.contributor.authorMonteiro, Jhonatas S.
dc.contributor.authorGrott, Suelen Cristina
dc.contributor.authorAlves, Thiago Caique
dc.contributor.authorTaniguchi, Satie
dc.contributor.authorBícego, Márcia Caruso
dc.contributor.authorde Almeida, Eduardo Alves
dc.contributor.authorBebianno, Maria João
dc.contributor.authorMedeiros, Igor D.
dc.contributor.authorBainy, Afonso C.D.
dc.date.accessioned2020-04-03T09:24:59Z
dc.date.available2020-04-03T09:24:59Z
dc.date.issued2019
dc.description.abstractOcean acidification is a result of the decrease in the pH of marine water, caused mainly by the increase in CO2 released in the atmosphere and its consequent dissolution in seawater. These changes can be dramatic for marine organisms especially for oysters Crassostrea gasar if other stressors such as xenobiotics are present. The effect of pH changes (6.5, 7.0 and 8.2) was assessed on the transcript levels of biotransformation [cytochromes P450 (CYP2AU1, CYP2-like2) and glutathione S-transferase (GSTΩ-like)] and antioxidant [superoxide dismutase (SOD-like), catalase (CAT-like) and glutathione peroxidase (GPx-like)] genes, as well as enzyme activities [superoxide dismutase, (SOD), catalase (CAT), glutathione reductase (GR), glutathione-S-transferases transferase (GST) and glucose-6-phosphate dehydrogenase (G6PDH)] and lipid peroxidation (MDA) in the gills of Crassostrea gasar exposed to 100 μg·L-1 of phenanthrene (PHE) for 24 and 96 h. Likewise, the PHE burdens was evaluated in whole soft tissues of exposed oysters. The accumulation of PHE in oysters was independent of pH. However, acidification promoted a significant decrease in the transcript levels of some protective genes (24 h exposure: CYP2AU1 and GSTΩ-like; 96 h exposure: CAT-like and GPx-like), which was not observed in the presence of PHE. Activities of GST, CAT and SOD enzymes increased in the oysters exposed to PHE at the control pH (8.2), but at a lower pH values, this activation was suppressed, and no changes were observed in the G6PDH activity and MDA levels. Biotransformation genes showed better responses after 24 h, and antioxidant-coding genes after 96 h, along with the activities of antioxidant enzymes (SOD, CAT), probably because biotransformation of PHE increases the generation of reactive oxygen species. The lack of change in MDA levels suggests that antioxidant modulation efficiently prevented oxidative stress. The effect of pH on the responses to PHE exposure should be taken into account before using these and any other genes as potential molecular biomarkers for PHE exposure.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.scitotenv.2019.04.450pt_PT
dc.identifier.issn0048-9697
dc.identifier.urihttp://hdl.handle.net/10400.1/13684
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S004896971932008X?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectCrassostreapt_PT
dc.subjectHydrogen-ion concentrationpt_PT
dc.subjectPhenanthrenespt_PT
dc.subjectProtonspt_PT
dc.subjectRandom allocationpt_PT
dc.subjectSeawaterpt_PT
dc.subjectStresspt_PT
dc.subjectTime factorspt_PT
dc.subjectWater pollutantspt_PT
dc.titleStress responses in Crassostrea gasar exposed to combined effects of acute pH changes and phenanthrenept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMAR%2F00350%2F2013/PT
oaire.citation.endPage593pt_PT
oaire.citation.startPage585pt_PT
oaire.citation.titleScience of the Total Environmentpt_PT
oaire.citation.volume678pt_PT
oaire.fundingStream5876
person.familyNameBebianno
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isProjectOfPublication3c9090f1-c8fc-4990-8cd8-d61d72eb13f7
relation.isProjectOfPublication.latestForDiscovery3c9090f1-c8fc-4990-8cd8-d61d72eb13f7

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