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Osteocalcin and Matrix Gla Protein in zebrafish (Danio rerio) and Senegal sole (Solea senegalensis): comparative gene and protein expression during larval development through adulthood

2006Journal article Unknown
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dc.contributor.authorGavaia, Paulo J.
dc.contributor.authorSimes, D
dc.contributor.authorOrtiz-Delgado, J. B.
dc.contributor.authorS B Viegas, Carla
dc.contributor.authorPinto, Jorge
dc.contributor.authorKelsh, R. N.
dc.contributor.authorSarasquete, C.
dc.contributor.authorCancela, Leonor
dc.date.accessioned2014-06-25T13:24:51Z
dc.date.available2014-06-25T13:24:51Z
dc.date.issued2006
dc.date.updated2014-05-30T10:23:12Z
dc.description.abstractBone Gla protein (Bgp or osteocalcin) and matrix Gla protein (Mgp) are important in calcium metabolism and skeletal development, but their precise roles at the molecular level remain poorly understood. Here, we compare the tissue distribution and accumulation of Bgp and Mgp during larval development and in adult tissues of zebrafish (Danio rerio) and throughout metamorphosis in Senegal sole (Solea senegalensis), two fish species with contrasting environmental calcium levels and degrees of skeletal reorganization at metamorphosis. Mineral deposition was investigated in parallel using a modified Alizarin red/Alcian blue protocol allowing sensitive simultaneous detection of bone and cartilage. In zebrafish, bgp and mgp mRNAs were localized in all mineralized tissues during and after calcification including bone and calcified cartilage of branchial arches. Through immunohistochemistry we demonstrated that these proteins accumulate mainly in the matrix of skeletal structures already calcified or under calcification, confirming in situ hybridization results. Interestingly, some accumulation of Bgp was also observed in kidney, possibly due to the presence of a related protein, nephrocalcin. Chromosomal localization of bgp and mgp using a zebrafish radiation hybrid panel indicated that both genes are located on the same chromosome, in contrast to mammals where they map to different chromosomes, albeit in regions showing synteny with the zebrafish location. Results in Senegal sole further indicate that, during metamorphosis, there is an increase in expression of both bgp and mgp, paralleling calcification of axial skeleton structures. In contrast with results obtained for previously studied marine fishes, in zebrafish and Senegal sole Mgp accumulates in both calcified tissues and non-mineralized vessel walls of the vascular system. These results suggest different patterns of Mgp accumulation between fish and mammals.por
dc.description.sponsorshipMCYT/AGL2003-03558
dc.identifier.citationGavaia, P.J., Simes, D.C., Viegas C.S.B., Pinto J., Ortiz-Delgado J.B., Kelsh R.N., Sarasquete M.C., Cancela M.L. 2006. Osteocalcin and Matrix Gla Protein in zebrafish (Danio rerio) and Senegal sole (Solea senegalensis): comparative gene and protein expression during larval development through adulthood. Mechanisms of Development- Gene Expression Patterns 6(6):637-52.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.modgep.2005.11.010
dc.identifier.otherAUT: LCA00739; AUT: DSI00813;
dc.identifier.urihttp://hdl.handle.net/10400.1/4504
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationInvolvement of Gla proteins MGP and Osteocalcin in the processes of mineralization occurring in marine versus fresh water teleosts.
dc.relationFUNCTIONAL ANALYSIS OF OSTEOCALCIN BONE CLA PROTEIN OR BGP FROM BONY FISH DURING SKELETAL DEVELOPMENT
dc.relationSCREENING OF MUTANT ZEBRAFISH FOR MODIFICATIONS OF BONE GLA PROTEIN AND OTHER BONE-RELATED PROTEINS: CHROMOSOME MAPPING AND EFFECTS OF OVERAND MIS-EXPRESSION DURING EARLY DEVELOPMENT
dc.subjectDanio reriopor
dc.subjectSolea senegalensispor
dc.subjectBGPpor
dc.subjectOsteocalcinpor
dc.subjectSkeletal developmentpor
dc.subjectGene expressionpor
dc.subjectIn situ hybridizationpor
dc.subjectImmunolocalizationpor
dc.subjectBonepor
dc.subjectCartilagepor
dc.titleOsteocalcin and Matrix Gla Protein in zebrafish (Danio rerio) and Senegal sole (Solea senegalensis): comparative gene and protein expression during larval development through adulthoodpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInvolvement of Gla proteins MGP and Osteocalcin in the processes of mineralization occurring in marine versus fresh water teleosts.
oaire.awardTitleFUNCTIONAL ANALYSIS OF OSTEOCALCIN BONE CLA PROTEIN OR BGP FROM BONY FISH DURING SKELETAL DEVELOPMENT
oaire.awardTitleSCREENING OF MUTANT ZEBRAFISH FOR MODIFICATIONS OF BONE GLA PROTEIN AND OTHER BONE-RELATED PROTEINS: CHROMOSOME MAPPING AND EFFECTS OF OVERAND MIS-EXPRESSION DURING EARLY DEVELOPMENT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCTI%2FCVT%2F42098%2F2001/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCTI/PRAXIS XXI%2FBD%2F19665%2F99/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//PRAXIS XXI%2FBPD%2F20229%2F99/PT
oaire.citation.endPage652por
oaire.citation.startPage637por
oaire.citation.titleGene Expression Patternspor
oaire.citation.volume6por
oaire.fundingStreamPOCI
oaire.fundingStreamPOCTI
person.familyNameGavaia
person.familyNameSimes
person.familyNameViegas
person.familyNamePinto
person.familyNameCancela
person.givenNamePaulo
person.givenNameDina
person.givenNameCarla
person.givenNameJorge
person.givenNameM. Leonor
person.identifier.ciencia-idB619-FC16-D007
person.identifier.ciencia-idC414-F596-EEC6
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0002-5145-4753
person.identifier.orcid0000-0002-5765-3665
person.identifier.orcid0000-0003-1501-458X
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridA-6470-2011
person.identifier.ridN-2789-2014
person.identifier.ridN-6695-2014
person.identifier.ridJ-7827-2013
person.identifier.scopus-author-id6507104377
person.identifier.scopus-author-id7201884723
person.identifier.scopus-author-id8656310300
person.identifier.scopus-author-id8656187600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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