Repository logo
 
Publication

Estradiol and genistein effects on the sea bass (Dicentrarchus labrax) scales: transcriptome dataset

2019-12Journal article Open access
Simple item page
dc.contributor.authorPinto, Patricia IS
dc.contributor.authorAndrade, André
dc.contributor.authorThorne, Michael A.S.
dc.contributor.authorEstêvão, M. Dulce
dc.contributor.authorCanario, Adelino
dc.contributor.authorPower, Deborah
dc.date.accessioned2020-01-06T17:54:08Z
dc.date.available2020-01-06T17:54:08Z
dc.date.issued2019-12
dc.description.abstractFish scales are mineralized structures that play important roles in protection and mineral homeostasis. This tissue expresses multiple estrogen receptor subtypes and can be targeted by estrogens or estrogenic endocrine-disrupting compounds, but their effects are poorly explored. The transcriptome data here presented support the findings reported in the research article "Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier" [1]. Juvenile sea bass were exposed to estradiol and the phytoestrogen genistein for 1 and 5 days, by intraperitoneal injections, and the effects on scale transcript expression were analysed by RNA-seq using an Illumina Hi-seq 1500. The raw reads of the 30 libraries produced have been deposited in the NCBI-SRA database with the project accession number SRP102504. Mapping of RNA-seq reads against the sea bass reference genome using the Cufflinks/TopHat package identified 371 genes that had significant (FDR<0.05) differential expression with the estradiol or genistein treatments in relation to the control scales at each exposure time, 254 of which presented more than a 2-fold change in expression. The identity of the differentially expressed genes was obtained using both automatic and manual annotations against multiple public sequence databases and they were grouped according to their patterns of expression using hierarchical clustering and heat-maps. The biological processes and KEGG pathways most significantly affected by the estradiol and/or genistein treatments were identified using Cytoscape/ClueGO enrichment analyses.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPinto, PIS; Andrade, AR; Moreira, C; Zapater, C; Thorne, MAS; Santos, S; Dulce Estevao, MD; et al. 2019. "Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier". The Journal of Steroid Biochemistry and Molecular 195: 105448. 10.1016/j.jsbmb.2019.105448pt_PT
dc.identifier.doihttps://doi.org/10.1016/j.dib.2019.104587pt_PT
dc.identifier.issn2352-3409
dc.identifier.urihttp://hdl.handle.net/10400.1/13405
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationE2SCALE: Scaling-down estrogenic responses in fish
dc.relationCentre of Marine Sciences
dc.relationNOVEL REGULATORS OF FISH PUBERTY ONSET
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEstrogen-responsive genespt_PT
dc.subjectGenistein-responsive genespt_PT
dc.subjectFish scalept_PT
dc.subjectRNA-seqpt_PT
dc.subjectTranscriptomept_PT
dc.titleEstradiol and genistein effects on the sea bass (Dicentrarchus labrax) scales: transcriptome datasetpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleE2SCALE: Scaling-down estrogenic responses in fish
oaire.awardTitleCentre of Marine Sciences
oaire.awardTitleNOVEL REGULATORS OF FISH PUBERTY ONSET
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAAG-GLO%2F4003%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04326%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBPD%2F84033%2F2012/PT
oaire.citation.startPage104587pt_PT
oaire.citation.titleData in Briefpt_PT
oaire.citation.volume27pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
person.familyNamePinto
person.familyNameAlçada Baptista Rebelo de Andrade
person.familyNameEstêvão
person.familyNameCanario
person.familyNamePower
person.givenNamePatricia IS
person.givenNameAndré
person.givenNameMaria Dulce da Mota Antunes de Oliveira
person.givenNameAdelino
person.givenNameDeborah Mary
person.identifier643457
person.identifierI-4695-2014
person.identifier143624
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.ciencia-idF513-56ED-7088
person.identifier.ciencia-id881B-53E8-0DB1
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-7854-3898
person.identifier.orcid0000-0002-5900-8782
person.identifier.orcid0000-0002-7151-8363
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-3817-2013
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id10240774300
person.identifier.scopus-author-id8381590400
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication64bc526e-5281-44e5-91eb-5743436109f9
relation.isAuthorOfPublication44cc56db-1879-412b-86eb-8ce5465a427a
relation.isAuthorOfPublication67887883-2695-48cc-8221-aa46780ac143
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscovery64bc526e-5281-44e5-91eb-5743436109f9
relation.isProjectOfPublication66289c1f-16cd-4d25-b30f-1eb63d5ecd80
relation.isProjectOfPublicationf38dbd9d-3734-4f3c-8d74-dff8dcaa8674
relation.isProjectOfPublicationd77daeaa-4366-4364-98ca-5ce001169ea5
relation.isProjectOfPublication.latestForDiscovery66289c1f-16cd-4d25-b30f-1eb63d5ecd80

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
1-s2.0-S2352340919309424-main.pdf
Size:
762.38 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.46 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ESS2-Artigos (em revistas ou actas indexadas)
CCM2-Artigos (em revistas ou actas indexadas)