Repository logo
 
Publication

Isolation of Atlantic halibut pituitary hormones by continuous-elution electrophoresis followed by fingerprint identification, and assessment of growth hormone content during larval development

2007Journal article Restricted access
Simple item page
dc.contributor.authorEinarsdóttir, Ingibjörg E.
dc.contributor.authorAnjos, Liliana
dc.contributor.authorHildahl, Jon
dc.contributor.authorBjörnsson, Björn Thrandur
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-10-24T12:23:56Z
dc.date.available2014-10-24T12:23:56Z
dc.date.issued2007
dc.description.abstractContinuous-elution electrophoresis (CEE) has been applied to separate putative hormones from adult Atlantic halibut pituitaries. Soluble proteins were separated by size and charge on Model 491 Prep Cell (Bio-Rad), where the homogenate runs through a cylindrical gel, and protein fractions are collected as they elute from the matrix. Protein fractions were assessed by SDS–PAGE and found to contain purified proteins of molecular size from 10 to 33 kDa. Fractions containing proteins with molecular weights of approximately 21, 24, 28 and 32 kDa, were identified as putative growth hormone (GH), prolactin, somatolactin and gonadotropins, respectively. These were analyzed further by mass spectrometry and identified with peptide mass protein fingerprinting. The CEE technique was used successfully for purification of halibut GH with a 5% yield, and appears generally well suited to purify species-specific proteins often needed for research in comparative endocrinology, including immunoassay work. Thus, the GH obtained was subsequently used as standards and iodination label in a homologous radioimmunoassay, applied to analyze GH content through larval development in normally and abnormally metamorphosing larvae. As GH is mainly found in the pituitary, GH contents were analyzed in tissue extracts from the heads only. The pituitary GH content increases proportionally to increased larval weight from first feeding to metamorphic climax. No difference in relative GH content was found between normal and abnormal larvae and it still remains to be established if GH has a direct role in metamorphosis.por
dc.description.sponsorshipWe thank Heiðdís Smáradóttir and Arnar Jónsson at Fiskey Ltd., Iceland, for excellent logistic and scientific collaboration. This work has been carried out within the project ‘‘Arrested development: The Molecular and Endocrine Basis of Flatfish Metamorphosis’’ (Q5RS-2002-01192) with financial support from the Commission of the European Communities. However, it does not necessarily reflect the Commission’s views and in no way anticipates its future policy in this area. This project was further supported by the Swedish Council for Agricultural and Forestry Research (FORMAS) and Pluriannual funding to CCMAR by the Portuguese Science and Technology Council.por
dc.identifier.citationIngibjörg Eir Einarsdóttir , Liliana Anjos, Jon Hildahl, Björn Thrandur Björnsson, Deborah M. Power, "Isolation of Atlantic halibut pituitary hormones by continuous-elution electrophoresis followed by fingerprint identification, and assessment of growth hormone content during larval development" in General and Comparative Endocrinology 150 (2007) 355–363.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.ygcen.2006.09.010
dc.identifier.issn0016-6480
dc.identifier.otherAUT: DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/5475
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectFlatfishpor
dc.subjectHippoglossus hippoglossuspor
dc.subjectGrowth hormonepor
dc.subjectProlactinpor
dc.subjectSomatolactinpor
dc.subjectFingerprintingpor
dc.subjectLarval developmentpor
dc.titleIsolation of Atlantic halibut pituitary hormones by continuous-elution electrophoresis followed by fingerprint identification, and assessment of growth hormone content during larval developmentpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage363por
oaire.citation.issue2por
oaire.citation.startPage355por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume150por
person.familyNameAnjos Guerreiro
person.familyNamePower
person.givenNameLiliana Isabel Tomé
person.givenNameDeborah Mary
person.identifierAAK-3800-2020
person.identifier.ciencia-id6D18-2D1C-A45C
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-9474-522X
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id8575757700
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication721b5dca-ad42-40f8-9731-517eb5db009e
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscovery721b5dca-ad42-40f8-9731-517eb5db009e

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Isolation of Atlantic halibut pituitary hormones by continuous-elution electrophoresis followed by fingerprint identification, and assessment of growth hormone content during larval development.pdf
Size:
1.24 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.49 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)