Publication
Heterotrophy as a tool to overcome the long and costly autotrophic scale-up process for large scale production of microalgae
| dc.contributor.author | Barros, A. | |
| dc.contributor.author | Pereira, H. | |
| dc.contributor.author | Campos, J. | |
| dc.contributor.author | Marques, A. | |
| dc.contributor.author | Varela, J. | |
| dc.contributor.author | Silva, J. | |
| dc.date.accessioned | 2019-10-15T14:27:20Z | |
| dc.date.available | 2019-10-15T14:27:20Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Industrial scale-up of microalgal cultures is often a protracted step prone to culture collapse and the occurrence of unwanted contaminants. To solve this problem, a two-stage scale-up process was developed - heterotrophically Chlorella vulgaris cells grown in fermenters (1st stage) were used to directly inoculate an outdoor industrial autotrophic microalgal production unit (2nd stage). A preliminary pilot-scale trial revealed that C. vulgaris cells grown heterotrophically adapted readily to outdoor autotrophic growth conditions (1-m3 photobioreactors) without any measurable difference as compared to conventional autotrophic inocula. Biomass concentration of 174.5 g L-1, the highest value ever reported for this microalga, was achieved in a 5-L fermenter during scale-up using the heterotrophic route. Inocula grown in 0.2- and 5-m3 industrial fermenters with mean productivity of 27.54 ± 5.07 and 31.86 ± 2.87 g L-1 d-1, respectively, were later used to seed several outdoor 100-m3 tubular photobioreactors. Overall, all photobioreactor cultures seeded from the heterotrophic route reached standard protein and chlorophyll contents of 52.18 ± 1.30% of DW and 23.98 ± 1.57 mg g-1 DW, respectively. In addition to providing reproducible, high-quality inocula, this two-stage approach led to a 5-fold and 12-fold decrease in scale-up time and occupancy area used for industrial scale-up, respectively. | pt_PT |
| dc.description.sponsorship | Agência financiadora project FERMALG 017608 Fundacao para a Ciencia e a Tecnologia (FCT) UID/Multi/04326/2019 project FERMALG (AVISO) 32/SI/2015 | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.1038/s41598-019-50206-z | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/12826 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Nature Publishing Group | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.title | Heterotrophy as a tool to overcome the long and costly autotrophic scale-up process for large scale production of microalgae | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 1 | pt_PT |
| oaire.citation.startPage | 13935 | pt_PT |
| oaire.citation.title | Scientific Reports | pt_PT |
| oaire.citation.volume | 9 | pt_PT |
| person.familyName | Pereira | |
| person.familyName | Varela | |
| person.givenName | Hugo | |
| person.givenName | João | |
| person.identifier | 282781 | |
| person.identifier.ciencia-id | 331E-84FD-2F34 | |
| person.identifier.orcid | 0000-0002-1369-2099 | |
| person.identifier.orcid | 0000-0003-3101-693X | |
| person.identifier.rid | E-9360-2015 | |
| person.identifier.rid | M-4223-2013 | |
| person.identifier.scopus-author-id | 54080259900 | |
| person.identifier.scopus-author-id | 55741416000 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | ebcb2180-5235-4502-b562-cd1e7786e6f9 | |
| relation.isAuthorOfPublication | d339a9e7-bc32-49c4-ba48-a17343d40092 | |
| relation.isAuthorOfPublication.latestForDiscovery | ebcb2180-5235-4502-b562-cd1e7786e6f9 |