Repository logo
 
Publication

Sex-dependent and-independent transcriptional changes during haploid phase gametogenesis in the sugar kelp Saccharina latissima

2019Journal article Open access
Simple item page
dc.contributor.authorPearson, Gareth
dc.contributor.authorMartins, Neusa
dc.contributor.authorMadeira, Pedro M.
dc.contributor.authorSerrao, Ester
dc.contributor.authorBartsch, Inka
dc.date.accessioned2020-06-16T13:20:48Z
dc.date.available2020-06-16T13:20:48Z
dc.date.issued2019
dc.description.abstractIn haplodiplontic lineages, sexual reproduction occurs in haploid parents without meiosis. Although widespread in multicellular lineages such as brown algae (Phaeophyceae), haplodiplontic gametogenesis has been little studied at the molecular level. We addressed this by generating an annotated reference transcriptome for the gametophytic phase of the sugar kelp, Saccharina latissima. Transcriptional profiles of microscopic male and female gametophytes were analysed at four time points during the transition from vegetative growth to gametogenesis. Gametogenic signals resulting from a switch in culture irradiance from red to white light activated a core set of genes in a sex-independent manner, involving rapid activation of ribosome biogenesis, transcription and translation related pathways, with several acting at the post-transcriptional or post-translational level. Additional genes regulating nutrient acquisition and key carbohydrate-energy pathways were also identified. Candidate sex-biased genes under gametogenic conditions had potentially key roles in controlling female- and male-specific gametogenesis. Among these were several sex-biased or -specific E3 ubiquitin-protein ligases that may have important regulatory roles. Females specifically expressed several genes that coordinate gene expression and/or protein degradation, and the synthesis of inositol-containing compounds. Other female-biased genes supported parallels with oogenesis in divergent multicellular lineages, in particular reactive oxygen signalling via an NADPH-oxidase. Males specifically expressed the hypothesised brown algal sex-determining factor. Male-biased expression mainly involved upregulation of genes that control mitotic cell proliferation and spermatogenesis in other systems, as well as multiple flagella-related genes. Our data and results enhance genome-level understanding of gametogenesis in this ecologically and economically important multicellular lineage.pt_PT
dc.description.sponsorshipFCTprograms UID/Multi/04326/2019, GENEKELP-PTDC/MAR-EST/6053/2014,MARFOR-Biodiversa/0004/2015, FRH/BPD/122567/2016. Swedish Research Council Formas, Naturvardsverket French National Research Agency (ANR) Spanish Government, FRCT, German Research Foundation (DFG), Pew Marine Fellowship, STSM Grant from the COST Action "Phycomorph" FA1406pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1371/journal.pone.0219723pt_PT
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.1/14019
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPublic Library of Sciencept_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectComputational biologypt_PT
dc.subjectGametogenesispt_PT
dc.subjectHigh-throughput nucleotide sequencingpt_PT
dc.subjectMolecular sequence annotationpt_PT
dc.subjectPhaeophytapt_PT
dc.subjectSugarspt_PT
dc.subjectGene expression profilingpt_PT
dc.subjectHaploidypt_PT
dc.subjectTranscriptomept_PT
dc.titleSex-dependent and-independent transcriptional changes during haploid phase gametogenesis in the sugar kelp Saccharina latissimapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue9pt_PT
oaire.citation.startPagee0219723pt_PT
oaire.citation.titlePLoS ONEpt_PT
oaire.citation.volume14pt_PT
person.familyNamePearson
person.familyNameMadeira
person.familyNameSerrao
person.givenNameGareth Anthony
person.givenNamePedro
person.givenNameEster A.
person.identifier113536
person.identifierC-6686-2012
person.identifier.ciencia-id3315-9919-1A52
person.identifier.ciencia-id1D1A-4459-86D8
person.identifier.ciencia-id8E18-1746-6F5C
person.identifier.ciencia-id5B13-B26E-B1EC
person.identifier.orcid0000-0002-0768-464X
person.identifier.orcid0000-0003-4333-2905
person.identifier.orcid0000-0002-2802-0957
person.identifier.orcid0000-0003-1316-658X
person.identifier.ridJ-3911-2013
person.identifier.scopus-author-id55916875600
person.identifier.scopus-author-id7004093604
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication0a10c448-c7f5-4c3b-9488-3917c707e35e
relation.isAuthorOfPublication7a8c0acc-a718-46d5-98dc-a724f1266e09
relation.isAuthorOfPublicationf5258ef3-20ff-4994-8134-5931a15d3ecc
relation.isAuthorOfPublication45ccfe90-155c-4d6f-9e86-8f0fd064005f
relation.isAuthorOfPublication.latestForDiscovery45ccfe90-155c-4d6f-9e86-8f0fd064005f

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
file20.pdf
Size:
2.79 MB
Format:
Adobe Portable Document Format
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)