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Differential involvement of the three nuclear estrogen receptors during oogenesis in European sea bass (Dicentrarchus labrax)

2019-03Journal article Open access
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dc.contributor.authorZapater, Cinta
dc.contributor.authorMolés, Gregorio
dc.contributor.authorMuñoz, Iciar
dc.contributor.authorPinto, Patricia IS
dc.contributor.authorCanario, Adelino
dc.contributor.authorGómez, Ana
dc.date.accessioned2019-09-24T15:34:53Z
dc.date.available2020-03-01T01:30:12Z
dc.date.issued2019-03
dc.description.abstractEstrogens are involved in a wide range of processes in vertebrate reproduction through ligand activation of their specific cognate receptors. In most teleosts, three nuclear estrogen receptor subtypes have been identified (Esr1, Esr2a, and Esr2b). Differences in ligand binding affinity and seasonal expression patterns in reproductive tissues among these Esr subtypes suggest distinct roles during oogenesis, vitellogenesis, and spermatogenesis. This study focuses on the role of the Esr subtypes in European sea bass (Dicentrarchus labrax) oogenesis and their endocrine regulation. The coding genes of the three Esr subtypes are highly expressed in reproduction-related tissues such as pituitary, gonad, and liver. Quantification of esr1, esr2a, and esr2b expression in the ovary and liver during a whole reproductive cycle showed different patterns depending on stage and subtype, suggesting differential roles of the three receptors in the regulation of oogenesis and vitellogenesis. Esr2a and Esr2b also showed differences in transcriptional activity and ligand affinity when functionally characterized in HEK293 cells. Finally, for the first time in teleosts, the localization of the three Esr subtypes in ovarian follicles and their regulation by gonadotropins is described. Immunodetection of the receptors revealed different distribution patterns in follicular cells and various subcellular locations of the oocyte. Gonadotropin stimulation of ovarian follicles in different stages of vitellogenesis showed a consistent induction of esrb2b expression by Fsh. All together, these data reinforce the hypothesis that each estrogen receptor plays a specific role in oogenesis.pt_PT
dc.description.sponsorshipAgência financiadora Spanish Ministery of Economy and Competitivity AGL2015-67477-C2-1-R Portuguese Foundation for Science and Technology UID/Multi/04326/2013 SFRH/BPD/84033/2012 Generalitat Valenciana PROMETEOII/2014/051pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doihttps://doi.org/10.1093/biolre/ioy227
dc.identifier.issn0006-3363
dc.identifier.urihttp://hdl.handle.net/10400.1/12780
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Presspt_PT
dc.relation.publisherversionhttps://www.ncbi.nlm.nih.gov/pubmed/30371737pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEstrogenpt_PT
dc.subjectEstrogen receptorpt_PT
dc.subjectFemale reproductionpt_PT
dc.subjectOvarypt_PT
dc.subjectVitellogenesispt_PT
dc.subjectTeleostpt_PT
dc.subjectImmunolocalizationpt_PT
dc.subjectGonadotropinspt_PT
dc.subjectTranscriptional activationpt_PT
dc.titleDifferential involvement of the three nuclear estrogen receptors during oogenesis in European sea bass (Dicentrarchus labrax)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F84033%2F2012/PT
oaire.citation.endPage772pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage757pt_PT
oaire.citation.titleBiology of Reproductionpt_PT
oaire.citation.volume100pt_PT
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNamePinto
person.familyNameCanario
person.givenNamePatricia IS
person.givenNameAdelino
person.identifier643457
person.identifier143624
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0001-7854-3898
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridM-3817-2013
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id10240774300
person.identifier.scopus-author-id56568523700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication64bc526e-5281-44e5-91eb-5743436109f9
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isProjectOfPublication868b4818-3efa-4edb-9202-c464d64fd38f
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