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Troponin T isoform expression is modulated during Atlantic Halibut metamorphosis

2007Journal article Open access
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dc.contributor.authorCampinho, M. A.
dc.contributor.authorSilva, Nadia
dc.contributor.authorNowell, Mari
dc.contributor.authorLlewellyn, Lynda
dc.contributor.authorSweeney, Glen E.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-10-24T09:17:48Z
dc.date.available2014-10-24T09:17:48Z
dc.date.issued2007
dc.description.abstractBackground: Flatfish metamorphosis is a thyroid hormone (TH) driven process which leads to a dramatic change from a symmetrical larva to an asymmetrical juvenile. The effect of THs on muscle and in particular muscle sarcomer protein genes is largely unexplored in fish. The change in Troponin T (TnT), a pivotal protein in the assembly of skeletal muscles sarcomeres and a modulator of calcium driven muscle contraction, during flatfish metamophosis is studied. Results: In the present study five cDNAs for halibut TnT genes were cloned; three were splice variants arising from a single fast TnT (fTnT) gene; a fourth encoded a novel teleost specific fTnTlike cDNA (AfTnT) expressed exclusively in slow muscle and the fifth encoded the teleost specific sTnT2. THs modified the expression of halibut fTnT isoforms which changed from predominantly basic to acidic isoforms during natural and T4 induced metamorphosis. In contrast, expression of red muscle specific genes, AfTnT and sTnT2, did not change during natural metamorphosis or after T4 treatment. Prior to and after metamorphosis no change in the dorso-ventral symmetry or temporal-spatial expression pattern of TnT genes and muscle fibre organization occurred in halibut musculature. Conclusion: Muscle organisation in halibut remains symmetrical even after metamorphosis suggesting TH driven changes are associated with molecular adaptations. We hypothesize that species specific differences in TnT gene expression in teleosts underlies different larval muscle developmental programs which better adapts them to the specific ecological constraints.por
dc.description.sponsorshipThis work was carried out within the project Q5RS-2002-01192 with financial support from the Commission of the European Communities. However, it does not necessarily reflect the Commission's views and in no way anticipates its future policy in this area. This project was further co-financed by POCI 2010 and the European social funds attributed by the Portuguese National Science Foundation (FCT) to a Pluriannual project to CCMAR and a PhD fellowship to MAC (SFRH/BD/6133/2001).por
dc.description.sponsorshipThis work was carried out within the project Q5RS-2002-01192 with financial support from the Commission of the European Communities. However, it does not necessarily reflect the Commission's views and in no way anticipates its future policy in this area. This project was further co-financed by POCI 2010 and the European social funds attributed by the Portuguese National Science Foundation (FCT) to a Pluriannual project to CCMAR and a PhD fellowship to MAC (SFRH/BD/6133/2001).
dc.identifier.citationMarco A Campinho, Nádia Silva, Mari A Nowell, Lynda Llewellyn, Glen E Sweeney and Deborah M Power, "Troponin T isoform expression is modulated during Atlantic Halibut metamorphosis" in BMC Developmental Biology 2007, 7:71por
dc.identifier.doihttp://dx.doi.org/ 10.1186/1471-213X-7-71
dc.identifier.issn1471-2148
dc.identifier.otherAUT: DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/5451
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherBMCpor
dc.relationPRINCÍPIOS MOLECULARES E ENDÓCRINOS NO DESENVOLVIMENTO E METAMORFOSE DO EMBRIÃO EM TELEÓSTEOS
dc.relation.publisherversionhttp://www.biomedcentral.com/1471-213X/7/71por
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectTroponin Tpor
dc.subjectMetamorphosipor
dc.titleTroponin T isoform expression is modulated during Atlantic Halibut metamorphosispor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePRINCÍPIOS MOLECULARES E ENDÓCRINOS NO DESENVOLVIMENTO E METAMORFOSE DO EMBRIÃO EM TELEÓSTEOS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F6133%2F2001/PT
oaire.citation.titleBMC Evolutionary Biologypor
oaire.citation.volume7por
person.familyNameCampinho
person.familyNameSilva
person.familyNamePower
person.givenNameMarco António
person.givenNameNádia
person.givenNameDeborah Mary
person.identifier.ciencia-id0E18-2560-6EC1
person.identifier.ciencia-id5D1F-6F3A-A1C2
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-5238-0506
person.identifier.orcid0000-0001-9881-4421
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridD-8833-2013
person.identifier.scopus-author-id8938999600
person.identifier.scopus-author-id10244362100
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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