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Oxyfluorfen: a novel metabolic inhibitor to select microalgal chlorophyll-deficient mutant strains for nutritional applications

2024-07Journal article Open access
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dc.contributor.authorTrovão dos Santos, Mafalda
dc.contributor.authorCardoso, Lucas
dc.contributor.authorSchüler, Lisa
dc.contributor.authorMachado, Adriana
dc.contributor.authorSanto, Gonçalo Espírito
dc.contributor.authorPedroso, Humberto
dc.contributor.authorReis, Ana
dc.contributor.authorBarros, Ana
dc.contributor.authorCorreia, Nádia
dc.contributor.authorCosta, Monya
dc.contributor.authorFerreira, Sara
dc.contributor.authorCardoso, Helena
dc.contributor.authorMateus, Marília
dc.contributor.authorSilva, Joana
dc.contributor.authorPereira, Hugo
dc.contributor.authorFreitas, Filomena
dc.contributor.authorVarela, João
dc.date.accessioned2024-09-24T08:38:32Z
dc.date.available2024-09-24T08:38:32Z
dc.date.issued2024-07
dc.description.abstractNowadays, there is an increasing demand for novel feedstocks and alternative protein sources to meet global needs. Because of their rich nutritional profiles and high protein contents, microalgae-based food products and supplements are being developed. Nonetheless, these products present organoleptic characteristics such as taste, smell and colour that are often considered unpleasant by human and animal consumers. To address this constraint, strain improvement approaches such as random mutagenesis have been used, which combined with the right selection strategy, lead to more appealing microalgal biomass. In this work, a novel selection strategy using oxyfluorfen, an inhibitor of the chlorophyll synthesis pathway, was applied for the first time to isolate chlorophyll-deficient strains of Scenedesmus rubescens and Chlorella vulgaris upon treatment with ethyl methanesulfonate (EMS). With this approach, one S. rubescens brownish (37Y01) mutant strain, as well as two C. vulgaris mutant strains, one yellow (31Y15) and one white (31W62), were obtained. S. rubescens 37Y01 displayed a reduced protein content of 19.1% dry weight (DW) compared to that of the wildtype, which presented a protein content of 25.0% DW. C. vulgaris wildtype and mutants exhibited higher protein contents, in the 42.844.3% DW range, compared to Scenedesmus rubescens (p < 0.05). The selective pressure of this inhibitor allowed the selection of S. rubescens and C. vulgaris mutants displaying 55% and 95% decrease in chlorophyll content, respectively. The reduced chlorophyll content greatly improves the sensory properties and consumer acceptance of established mutants, increasing the potential of both strains as feedstocks to develop novel food products.eng
dc.description.sponsorshipALG-01-0247-FEDER-069961; C644915664-00000026; 2020.06814; 2020.07229
dc.identifier.doi10.1016/j.algal.2024.103572
dc.identifier.issn2211-9264
dc.identifier.urihttp://hdl.handle.net/10400.1/25921
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationMICROALGAE PROTEIN INGREDIENTS FOR THE FOOD AND FEED OF THE FUTURE
dc.relationApplied Molecular Biosciences Unit
dc.relationApplied Molecular Biosciences Unit
dc.relationInstitute for Health and Bioeconomy
dc.relationCentre for Marine and Environmental Research
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relation.ispartofAlgal Research
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectMicroalgae
dc.subjectHeterotrophic
dc.subjectStrain improvement
dc.subjectRandom mutagenesis
dc.subjectSelection method
dc.subjectChlorophyll
dc.subjectProtein
dc.titleOxyfluorfen: a novel metabolic inhibitor to select microalgal chlorophyll-deficient mutant strains for nutritional applicationseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMICROALGAE PROTEIN INGREDIENTS FOR THE FOOD AND FEED OF THE FUTURE
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleInstitute for Health and Bioeconomy
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/862980/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0140%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.citation.startPage103572
oaire.citation.titleAlgal Research:Biomass, Biofuels and Bioproducts
oaire.citation.volume81
oaire.fundingStreamH2020
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameTrovão dos Santos
person.familyNameSchüler
person.familyNameCosta
person.familyNamePereira
person.familyNameVarela
person.givenNameMafalda
person.givenNameLisa
person.givenNameMonya
person.givenNameHugo
person.givenNameJoão
person.identifier282781
person.identifier.ciencia-idD912-559A-7706
person.identifier.ciencia-idE01F-9B43-4F24
person.identifier.ciencia-id331E-84FD-2F34
person.identifier.orcid0000-0002-9465-7681
person.identifier.orcid0000-0003-0961-5397
person.identifier.orcid0000-0002-4236-9063
person.identifier.orcid0000-0002-1369-2099
person.identifier.orcid0000-0003-3101-693X
person.identifier.ridE-9360-2015
person.identifier.ridM-4223-2013
person.identifier.scopus-author-id8094380800
person.identifier.scopus-author-id54080259900
person.identifier.scopus-author-id55741416000
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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