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Cloning of the glucocorticoid receptor (GR) in gilthead seabream (Sparus aurata)

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dc.contributor.authorAcerete, L.
dc.contributor.authorBalasch, J. C.
dc.contributor.authorCastellana, B.
dc.contributor.authorRedruello, Begoña
dc.contributor.authorRoher, N.
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorPlanas, J. V.
dc.contributor.authorMacKenzie, S.
dc.contributor.authorTort, L.
dc.date.accessioned2014-05-09T15:11:22Z
dc.date.available2014-05-09T15:11:22Z
dc.date.issued2007
dc.date.updated2014-05-08T11:41:02Z
dc.description.abstractIn order to determine the cortisol response after an immune challenge in the gilthead seabream (Sparus aurata), a cortisol receptor (GR) was cloned, sequenced and its expression determined after lipopolysaccharide (LPS) treatment. To clone the gilthead seabream GR (sbGR), consecutive PCR amplifications and screening of a pituitary cDNA library were performed. We obtained a clone of 4586 bp encoding a 784aa protein. Northern blot analysis from head kidney, heart and intestine revealed that the full length sbGR mRNA was approximately 6.5 Kb. A LPS treatment, used as an acute stress model, was employed to characterise the expression of sbGR and some selected genes involved in the immune response (IL-1β, TNF-α, Mx protein, cathepsin D and PPAR-γ). All genes were expressed in all tissues examined and responses were tissue and time dependent revealing differential gene expression profiles after LPS administration. Furthermore, analysis of plasma cortisol levels after LPS injection, showed an acute response to inflammatory stress with a significant increase two and six h after injection, recovering to basal levels 12 h post-stress in all LPS concentrations tested.por
dc.identifier.citationAcerete, L.; Balasch, J.C.; Castellana, B.; Redruello, B.; Roher, N.; Canario, A.V.; Planas, J.V.; MacKenzie, S.; Tort, L. Cloning of the glucocorticoid receptor (GR) in gilthead seabream (Sparus aurata), Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 148, 1, 32-43, 2007.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.cbpb.2007.04.015
dc.identifier.issn1095-6433
dc.identifier.otherAUT: ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3837
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectEndotoxinpor
dc.subjectStresspor
dc.subjectSparus auratapor
dc.subjectImmunepor
dc.subjectGlucocorticoid receptorpor
dc.titleCloning of the glucocorticoid receptor (GR) in gilthead seabream (Sparus aurata)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage43por
oaire.citation.issue1por
oaire.citation.startPage32por
oaire.citation.titleComparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biologypor
oaire.citation.volume148por
person.familyNameRedruello
person.familyNameCanario
person.givenNameBegoña
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0003-1787-5594
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication67798ebb-16f5-4974-8d52-5158ddf9fe53
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery67798ebb-16f5-4974-8d52-5158ddf9fe53

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