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Phylogenetically and spatially close marine sponges harbour divergent bacterial communities

2012Journal article Open access
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dc.contributor.authorHardoim, C. C. P.
dc.contributor.authorEsteves, A. I. S.
dc.contributor.authorPires, F. R.
dc.contributor.authorGonçalves, J. M. S.
dc.contributor.authorCox, C. J.
dc.contributor.authorXavier, J.
dc.contributor.authorda Silva Costa, Rodrigo
dc.date.accessioned2014-10-27T15:24:22Z
dc.date.available2014-10-27T15:24:22Z
dc.date.issued2012
dc.date.updated2014-10-24T10:05:39Z
dc.description.abstractRecent studies have unravelled the diversity of sponge-associated bacteria that may play essential roles in sponge health and metabolism. Nevertheless, our understanding of this microbiota remains limited to a few host species found in restricted geographical localities, and the extent to which the sponge host determines the composition of its own microbiome remains a matter of debate. We address bacterial abundance and diversity of two temperate marine sponges belonging to the Irciniidae family - Sarcotragus spinosulus and Ircinia variabilis – in the Northeast Atlantic. Epifluorescence microscopy revealed that S. spinosulus hosted significantly more prokaryotic cells than I. variabilis and that prokaryotic abundance in both species was about 4 orders of magnitude higher than in seawater. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) profiles of S. spinosulus and I. variabilis differed markedly from each other – with higher number of ribotypes observed in S. spinosulus – and from those of seawater. Four PCR-DGGE bands, two specific to S. spinosulus, one specific to I. variabilis, and one present in both sponge species, affiliated with an uncultured sponge-specific phylogenetic cluster in the order Acidimicrobiales (Actinobacteria). Two PCR-DGGE bands present exclusively in S. spinosulus fingerprints affiliated with one sponge-specific phylogenetic cluster in the phylum Chloroflexi and with sponge-derived sequences in the order Chromatiales (Gammaproteobacteria), respectively. One Alphaproteobacteria band specific to S. spinosulus was placed in an uncultured sponge-specific phylogenetic cluster with a close relationship to the genus Rhodovulum. Our results confirm the hypothesized host-specific composition of bacterial communities between phylogenetically and spatially close sponge species in the Irciniidae family, with S. spinosulus displaying higher bacterial community diversity and distinctiveness than I. variabilis. These findings suggest a pivotal host-driven effect on the shape of the marine sponge microbiome, bearing implications to our current understanding of the distribution of microbial genetic resources in the marine realm.por
dc.description.sponsorshipThis work was financed by the Portuguese Foundation for Science and Technology (FCT - http://www.fct.pt) through the research project PTDC/MAR/101431/2008. CCPH has a PhD fellowship granted by FCT (Grant No. SFRH/BD/60873/2009). JRX’s research is funded by a FCT postdoctoral fellowship (grant no. SFRH/BPD/62946/2009). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.por
dc.identifier.citationHardoim, C.C.P.; Esteves, A.I.S.; Pires, F.R.; Gonçalves, J.M.S.; Cox, C.J.; Xavier, J.R.; Costa, R. Phylogenetically and Spatially Close Marine Sponges Harbour Divergent Bacterial Communities, PLoS ONE, 7, 12, 1-15, 2012.por
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0053029
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.1/5534
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherPublic Library of Sciencepor
dc.titlePhylogenetically and spatially close marine sponges harbour divergent bacterial communitiespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage15por
oaire.citation.issue12por
oaire.citation.startPage1por
oaire.citation.titlePLoS ONEpor
oaire.citation.volume7por
person.familyNameGonçalves
person.familyNameCox
person.familyNameda Silva Costa
person.givenNameJorge Manuel Santos
person.givenNameCymon
person.givenNameRodrigo
person.identifier356846
person.identifier115920
person.identifier.ciencia-id1013-ED6A-65EB
person.identifier.ciencia-id6B15-9771-1D04
person.identifier.ciencia-id5917-D500-D251
person.identifier.orcid0000-0001-7704-8190
person.identifier.orcid0000-0002-4927-979X
person.identifier.orcid0000-0002-5932-4101
person.identifier.ridG-5206-2011
person.identifier.ridD-1303-2012
person.identifier.ridN-7274-2013
person.identifier.scopus-author-id7103326116
person.identifier.scopus-author-id7402112716
person.identifier.scopus-author-id7203063627
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication.latestForDiscovery4495127c-16f2-4231-9fc1-b471c661036b

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