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Genomic blueprints of sponge-prokaryote symbiosis are shared by low abundant and cultivatable Alphaproteobacteria

2019-02-13Journal article Open access
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dc.contributor.authorKarimi, Elham
dc.contributor.authorKeller-Costa, Tina
dc.contributor.authorSlaby, Beate M
dc.contributor.authorCox, Cymon
dc.contributor.authorda Rocha, Ulisses N
dc.contributor.authorHentschel, Ute
dc.contributor.authorda Silva Costa, Rodrigo
dc.date.accessioned2019-02-27T10:45:47Z
dc.date.available2019-02-27T10:45:47Z
dc.date.issued2019-02-13
dc.description.abstractMarine sponges are early-branching, filter-feeding metazoans that usually host complex microbiomes comprised of several, currently uncultivatable symbiotic lineages. Here, we use a low-carbon based strategy to cultivate low-abundance bacteria from Spongia officinalis. This approach favoured the growth of Alphaproteobacteria strains in the genera Anderseniella, Erythrobacter, Labrenzia, Loktanella, Ruegeria, Sphingorhabdus, Tateyamaria and Pseudovibrio, besides two likely new genera in the Rhodobacteraceae family. Mapping of complete genomes against the metagenomes of S. officinalis, seawater, and sediments confirmed the rare status of all the above-mentioned lineages in the marine realm. Remarkably, this community of low-abundance Alphaproteobacteria possesses several genomic attributes common to dominant, presently uncultivatable sponge symbionts, potentially contributing to host fitness through detoxification mechanisms (e.g. heavy metal and metabolic waste removal, degradation of aromatic compounds), provision of essential vitamins (e.g. B6 and B12 biosynthesis), nutritional exchange (especially regarding the processing of organic sulphur and nitrogen) and chemical defence (e.g. polyketide and terpenoid biosynthesis). None of the studied taxa displayed signs of genome reduction, indicative of obligate mutualism. Instead, versatile nutrient metabolisms along with motility, chemotaxis, and tight-adherence capacities - also known to confer environmental hardiness - were inferred, underlying dual host-associated and free-living life strategies adopted by these diverse sponge-associated Alphaproteobacteria.pt_PT
dc.description.sponsorshipPTDC/MAR-BIO/1547/2014; full PhD scholarship from the Erasmus Mundus Programme/SALAM EMA2 lot7/SALA1206422pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1038/s41598-019-38737-xpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.1/12376
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Publishing Grouppt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSponge-prokaryotept_PT
dc.subjectAlphaproteobacteriapt_PT
dc.titleGenomic blueprints of sponge-prokaryote symbiosis are shared by low abundant and cultivatable Alphaproteobacteriapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-MIC%2F3865%2F2012/PT
oaire.citation.endPage15
oaire.citation.issue1pt_PT
oaire.citation.startPage1999pt_PT
oaire.citation.startPage1
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume9pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameKarimi
person.familyNameKeller-Costa
person.familyNameCox
person.familyNameda Silva Costa
person.givenNameElham
person.givenNameTina
person.givenNameCymon
person.givenNameRodrigo
person.identifier458972
person.identifier115920
person.identifier.ciencia-id9319-A7D5-5335
person.identifier.ciencia-id6B15-9771-1D04
person.identifier.ciencia-id5917-D500-D251
person.identifier.orcid0000-0002-2204-0789
person.identifier.orcid0000-0003-3702-9192
person.identifier.orcid0000-0002-4927-979X
person.identifier.orcid0000-0002-5932-4101
person.identifier.ridD-1303-2012
person.identifier.ridN-7274-2013
person.identifier.scopus-author-id7402112716
person.identifier.scopus-author-id7203063627
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery82c3689c-60b6-440d-9d7b-49e6dbd6861b
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