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Methylation status of the telomerase reverse transcriptase promoter in parotid tumours and adjacent parotid gland tissue: a pilot study on the implications for recurrence and development of malignancy

2025-05-28Journal article Open access
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datacite.subject.sdg03:Saúde de Qualidade
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
datacite.subject.sdg04:Educação de Qualidade
dc.contributor.authorPaiva Correia, Antonio
dc.contributor.authorApolónio, Joana
dc.contributor.authorNadal, Alfons
dc.contributor.authorBrandão, José Ricardo
dc.contributor.authorSilva, Nádia
dc.contributor.authorMachado, Bianca
dc.contributor.authorArchilla, Ivan
dc.contributor.authorCastelo-Branco, Pedro
dc.contributor.authorHellquist, Henrik
dc.date.accessioned2026-01-12T11:43:28Z
dc.date.available2026-01-12T11:43:28Z
dc.date.issued2025-05-28
dc.description.abstractBackground/Objectives: The methylation of the hypermethylated oncological region (THOR) of human telomerase reverse transcriptase (hTERT) may forecast tumour aggressiveness. This pilot study aimed to evaluate THOR methylation as a potential biomarker for recurrence/malignant transformation in salivary gland pleomorphic adenomas (PA). Methods: THOR methylation was assessed by quantitative pyrosequencing in 96 parotid tissue samples (benign and malignant), including non-neoplastic parotid tissue, PA, recurrent PA (rPA), and carcinomas, along with their adjacent tissues. TERT promoter mutations (TPMs) were analysed by Sanger sequencing. Results: THOR methylation significantly differed across the seven groups. Malignant tissues showed higher THOR methylation than non-neoplastic tissues, whereas benign tumours showed no significant difference from non-neoplastic tissue. THOR methylation in rPA was closer to carcinoma than to normal tissue, similar in rPA and tissues adjacent to rPA, and higher in tissues adjacent to carcinomas than in non-neoplastic tissues. A subset of PA-adjacent tissues showed epigenetic alterations, suggesting an increased risk of recurrence or malignant transformation (5–15%). No TPMs were detected. Conclusions: THOR methylation may add information to differentiate normal from carcinogenic tissues and, as such, may be included in a biomarkers panel. Epigenetic alterations in PA-adjacent tissues with normal histology highlight the need for improved diagnostic markers.eng
dc.description.sponsorshipCAC/0007/2022
dc.identifier.doi10.3390/curroncol32060312
dc.identifier.issn1718-7729
dc.identifier.urihttp://hdl.handle.net/10400.1/28077
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relation.ispartofCurrent Oncology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSalivary gland neoplasms
dc.subjectCancer
dc.subjectPleomorphic adenoma
dc.subjectHTERT
dc.subjectTHOR
dc.subjectMethylation
dc.subjectBiomarkers
dc.titleMethylation status of the telomerase reverse transcriptase promoter in parotid tumours and adjacent parotid gland tissue: a pilot study on the implications for recurrence and development of malignancyeng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue6
oaire.citation.titleCurrent Oncology
oaire.citation.volume32
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNamePaiva Correia
person.familyNameApolónio
person.familyNameSilva
person.familyNameCastelo-Branco
person.familyNameHellquist
person.givenNameAntonio
person.givenNameJoana
person.givenNameNádia
person.givenNamePedro
person.givenNameHenrik
person.identifier.ciencia-id791E-9E39-2795
person.identifier.ciencia-id5D1F-6F3A-A1C2
person.identifier.ciencia-idE015-7F8F-5CA1
person.identifier.ciencia-id9C11-221B-93BF
person.identifier.orcid0000-0001-8725-0202
person.identifier.orcid0000-0002-9727-1813
person.identifier.orcid0000-0001-9881-4421
person.identifier.orcid0000-0002-3453-3978
person.identifier.orcid0000-0003-3044-6065
person.identifier.scopus-author-id10244362100
relation.isAuthorOfPublicationf8203149-8981-4f75-9463-afb5badb2122
relation.isAuthorOfPublication01cf2be1-ea9f-4082-bcc6-e50fe4ad9fe1
relation.isAuthorOfPublicationa467188c-1363-4f77-a9a9-8297de10a01a
relation.isAuthorOfPublicationbb25b5ad-1769-42be-a7d3-8fe76215aa23
relation.isAuthorOfPublicatione632b82a-cf09-4f9a-b445-9d9a9de47438
relation.isAuthorOfPublication.latestForDiscoveryf8203149-8981-4f75-9463-afb5badb2122

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