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Repurposing bacterial lysates: Engineering inhalable locust bean gum microparticles for respiratory infection prevention

2025-12Artigo científico Acesso aberto
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datacite.subject.sdg03:Saúde de Qualidade
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorda Silva, Joana Pinto
dc.contributor.authorBerzosa, Melibea
dc.contributor.authorChiarentin, Lucas
dc.contributor.authorDelgado-López, Alberto
dc.contributor.authorAlmeida, Maria Patricia
dc.contributor.authorVitorino, Carla
dc.contributor.authorFigueiras, Ana
dc.contributor.authorRosa da Costa, Ana M
dc.contributor.authorGamazo, Carlos
dc.contributor.authorGrenha, Ana
dc.date.accessioned2026-03-09T13:23:23Z
dc.date.available2026-03-09T13:23:23Z
dc.date.issued2025-12
dc.description.abstractThis study explores the development of inhalable microparticles containing bacterial lysates (BL) from multiple relevant bacterial species that include Staphylococcus aureus, Streptococcus pyogenes and Klebsiella pneumoniae, and locust bean gum (LBG) for pulmonary immunization against respiratory infections. LBG, a galactomannan, was chosen for its mucoadhesive properties and affinity for antigen-presenting cells. Microparticles were prepared by spray-drying, testing different LBG:BL ratios. The morphological analysis revealed convoluted microparticles, and BL association efficiency up to 81 % was determined. Antigenic assays confirmed that bacterial antigens of S. pyogenes, used as reference, remained preserved on the microparticles despite the applied processing conditions. The aerodynamic analysis showed that lower BL content (LBG:BL = 10:0.2, w/w) produced more suitable particles for pulmonary delivery, with 4.6 μm mass median aerodynamic diameter (MMAD) and 29 % fine particle fraction (FPF). Fluorescence microscopy confirmed uniform distribution of BL within the LBG matrix and LBG microparticles demonstrated superior adhesive properties compared to controls. Sustained release of BL from microparticles was observed in vitro, reaching around 80 % after 6 h, which increased to around 85 % after 24 h. Cytotoxicity studies showed appropriate cell viability at physiologically relevant concentrations (around 70 % or more). These findings suggest that LBG-based microparticles loaded with BL have potential to be explored as inhalable formulation for prevention of respiratory infections, offering targeted delivery and prolonged antigen presentation to enhance immune responses.eng
dc.description.sponsorshipUID/04326/2025; UID/PRR/04326/2025; PTDC/NAN-MAT/1431/2021
dc.identifier.doi10.1016/j.ijbiomac.2025.149097
dc.identifier.issn0141-8130
dc.identifier.urihttp://hdl.handle.net/10400.1/28383
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationCentre for Marine and Environmental Research
dc.relationCoimbra Chemistry Center
dc.relationCoimbra Chemistry Center
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBacterial lysates
dc.subjectInhalation
dc.subjectLocust bean gum
dc.subjectMicroparticles
dc.subjectSpray-drying
dc.titleRepurposing bacterial lysates: Engineering inhalable locust bean gum microparticles for respiratory infection preventioneng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberLA/P/0101/2020
oaire.awardNumberUIDB/00313/2020
oaire.awardNumberUIDP/00313/2020
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardTitleCoimbra Chemistry Center
oaire.awardTitleCoimbra Chemistry Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00313%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00313%2F2020/PT
oaire.citation.startPage149097
oaire.citation.titleInternational Journal of Biological Macromolecules
oaire.citation.volume334
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameda Silva
person.familyNameAlmeida
person.familyNameRosa da Costa
person.familyNameGrenha
person.givenNameJoana Pinto
person.givenNameMaria Patricia
person.givenNameAna M
person.givenNameAna
person.identifier305029
person.identifier.ciencia-id9318-BAE4-B025
person.identifier.ciencia-idA418-A85E-5DB1
person.identifier.ciencia-id091C-0D58-7225
person.identifier.orcid0000-0002-7706-1544
person.identifier.orcid0009-0001-9018-424X
person.identifier.orcid0000-0003-0225-9537
person.identifier.orcid0000-0002-2136-1396
person.identifier.ridE-2165-2012
person.identifier.ridH-1392-2017
person.identifier.scopus-author-id53986075100
person.identifier.scopus-author-id8607930100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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