In vitro morphogenesis in zygotic embryo cultures of carob tree (Ceratonia siliqua L.)

Custódio, Luísa; Romano, Anabela

http://hdl.handle.net/10400.1/6430

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
In vitro morphogenesis in zygotic embryo cultures of carob tree (Ceratonia siliqua L.).pdf		624.06 KB	Adobe PDF	Download

Send Feedback

Authors

Custódio, Luísa

Romano, Anabela

Abstract(s)

In this study, organogenic cultures were induced from zygotic embryos of carob tree. Basal medium consisted of Murashige & Tucker (1969), supplemented with 0.5 or 1 mg L-1 of 2,4-dichlorophenoxyacetic acid alone or combined with 0.5, 1, 2, 3 and 4 mg L-1 of kinetin. Calli initiation occurred in all the hormonal combinations tested. The zygotic embryo cultures differentiated two types of regenerants: somatic embryos (SEs) and shoots, with a maximum frequency of 30 and 100%, respectively. SEs exhibited morphological abnormalities, such as fusion and absence of cotyledons, and showed secondary embryogenesis. The regenerated shoots were rooted, hardened and transferred to the soil.