Repository logo
 
Publication

Batch studies on the biodegradation of paracetamol and 1,4-hydroquinone by novel bacterial strains isolated from extreme environmental samples and the identification of candidate catabolic genes

2024-08-13Journal article Open access
Simple item page
dc.contributor.authorLara-Moreno, Alba
dc.contributor.authorFatma El-Sayed
dc.contributor.authorCox, Cymon
dc.contributor.authorCosta, Maria Clara
dc.contributor.authorCarlier, Jorge
dc.date.accessioned2024-10-30T09:54:02Z
dc.date.available2024-10-30T09:54:02Z
dc.date.issued2024-08-13
dc.description.abstractThe emerging pollutant paracetamol (APAP) is one of the most prescribed drugs worldwide. In addition, APAP and its main metabolites, namely, 4-aminophenol (4-AP), hydroquinone (H2Q), benzoquinone (BQ), and 2,5-dihydroxy-1,4-benzoquinone (2,5-OH-BQ), among others, are frequently detected in wastewater treatment plants (WWTPs) influents, effluents, and the environment. Thus, continuous release into the environment, especially aquatic environments, is a source of general concern. Six APAP-degrading bacterial strains were isolated from two mine samples from the Iberian Pyrite Belt (Lousal and Poderosa mines). Mycolicibacterium aubagnense HPB1.1, which was isolated using enrichment cultures from the Poderosa mine sample in the presence of H2Q as the sole carbon source, also showed APAP biodegrading capabilities. Pure cultures of this strain degraded 34.3 mg L-1 of APAP in 5 days and 9.4 mg L-1 of H2Q in 4 days. Interestingly, BQ and 2,5-OH-BQ were detected as metabolites resulting from H2Q abiotic degradation, but these compounds were removed in the strain's cultures. Furthermore, M. aubagnense HPB1.1 whole-genome was sequenced, and its encoded proteins were aligned with enzymes of APAP-degrading bacteria recovered from databases and literature aiming to identify candidate catabolic genes. Putative amidases, deaminases, hydroxylases, and dioxygenases, responsible for the degradation of APAP by the HPB1.1 strain, were identified by similarity, corroborating its ability to transform APAP and its intermediate metabolite H2Q into less toxic metabolic compounds due to their capacity to break the aromatic ring of these molecules.eng
dc.identifier.doi10.1007/s13201-024-02264-6
dc.identifier.eissn2190-5495
dc.identifier.issn2190-5487
dc.identifier.urihttp://hdl.handle.net/10400.1/26174
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer Science and Business Media
dc.relationBioaugmentation and conjugative plasmid transference using bacteria from extreme environments to enhance biodegradation of recalcitrant pollutants in WWTP granular sludge
dc.relation.ispartofApplied Water Science
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBioremediation
dc.subjectAerobic biodegradation
dc.subjectWastewater treatment plants
dc.subjectMycolicibacterium aubagnense HPB1.1
dc.subjectEmerging contaminant
dc.subjectBiodegrading genes
dc.titleBatch studies on the biodegradation of paracetamol and 1,4-hydroquinone by novel bacterial strains isolated from extreme environmental samples and the identification of candidate catabolic geneseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBioaugmentation and conjugative plasmid transference using bacteria from extreme environments to enhance biodegradation of recalcitrant pollutants in WWTP granular sludge
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCTA-AMB%2F7782%2F2020/PT
oaire.citation.issue9
oaire.citation.startPage198
oaire.citation.titleApplied Water Science
oaire.citation.volume14
oaire.fundingStream3599-PPCDT
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameLara-Moreno
person.familyNameCox
person.familyNameCosta
person.familyNameCarlier
person.givenNameAlba
person.givenNameCymon
person.givenNameMaria Clara
person.givenNameJorge
person.identifier.ciencia-id6B15-9771-1D04
person.identifier.ciencia-id7110-A28E-3C8A
person.identifier.ciencia-id691D-A1BB-15CC
person.identifier.orcid0000-0002-5741-7662
person.identifier.orcid0000-0002-4927-979X
person.identifier.orcid0000-0003-1340-5237
person.identifier.orcid0000-0003-0675-2716
person.identifier.ridD-1303-2012
person.identifier.ridM-6189-2013
person.identifier.scopus-author-id7402112716
person.identifier.scopus-author-id35354952400
person.identifier.scopus-author-id7102166949
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication300c1ba7-9297-4afb-b2a1-3201af0cd639
relation.isAuthorOfPublication82c3689c-60b6-440d-9d7b-49e6dbd6861b
relation.isAuthorOfPublication11c29f10-6e2b-40d8-9c74-2cc45be6d7a7
relation.isAuthorOfPublicationd4af2b00-61d6-4fd9-b05d-9ed235b5c7de
relation.isAuthorOfPublication.latestForDiscovery82c3689c-60b6-440d-9d7b-49e6dbd6861b
relation.isProjectOfPublication0b09c039-bd11-41d7-bebf-ae11435c521d
relation.isProjectOfPublication.latestForDiscovery0b09c039-bd11-41d7-bebf-ae11435c521d

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Batch studies on the biodegradation of paracetamol.pdf
Size:
1.59 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.46 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)
FCT2-Artigos (em revistas ou actas indexadas)