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Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhua L.): cloning, tissue expression and comparative aspects

2007Journal article Restricted access
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dc.contributor.authorRønnestad, I.
dc.contributor.authorGavaia, Paulo J.
dc.contributor.authorS B Viegas, Carla
dc.contributor.authorVerri, T.
dc.contributor.authorNilsen, T. O.
dc.contributor.authorJordal, A. E. O.
dc.contributor.authorKamisaka, Y.
dc.contributor.authorCancela, Leonor
dc.date.accessioned2014-06-03T14:29:42Z
dc.date.available2014-06-03T14:29:42Z
dc.date.issued2007
dc.date.updated2014-05-30T10:11:59Z
dc.description.abstractA novel full-length cDNA that encodes for the Atlantic cod (Gadus morhua L.) PepT1-type oligopeptide transporter has been cloned. This cDNA (named codPepT1) was 2838·bp long, with an open reading frame of 2190·bp encoding a putative protein of 729 amino acids. Comparison of the predicted Atlantic cod PepT1 protein with zebrafish, bird and mammalian orthologs allowed detection of many structural features that are highly conserved among all the vertebrate proteins analysed, including (1) a larger than expected area of hydrophobic amino acids in close proximity to the N terminus; (2) a single highly conserved cAMP/cGMP-dependent protein kinase phosphorylation motif; (3) a large N-glycosylationrich region within the large extracellular loop; and (4) a conserved and previously undescribed stretch of 8–12 amino acid residues within the large extracellular loop. Expression analysis at the mRNA level indicated that Atlantic cod PepT1 is mainly expressed at intestinal level, but that it is also present in kidney and spleen. Analysis of its regional distribution along the intestinal tract of the fish revealed that PepT1 is ubiquitously expressed in all segments beyond the stomach, including the pyloric caeca, and through the whole midgut. Only in the last segment, which included the hindgut, was there a lower expression. Atlantic cod PepT1, the second teleost fish PepT1-type transporter documented to date, will contribute to the elucidation of the evolutionary and functional relationships among vertebrate peptide transporters. Moreover, it can represent a useful tool for the study of gut functional regionalization, as well as a marker for the analysis of temporal and spatial expression during ontogeny.por
dc.identifier.citationI. Ronnestad; Gavaia, Paulo Jorge Travessa; Carla S.B. Viegas; T. Verri; T.O Nilsen; A.E.O. Jordal; Y. Kamisaka; Leonor Cancela. Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhua L.): cloning, tissue expression and comparative aspects, J. Exp. Biol. 210, 22, 3883-3896, 2007.por
dc.identifier.doihttp://dx.doi.org/10.1242/jeb.007898
dc.identifier.issn0022-0949
dc.identifier.otherAUT: LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/4171
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherCompany of Biologistspor
dc.subjectOligopeptide transporter PepT1por
dc.subjectTissue expressionpor
dc.subjectPeptidepor
dc.subjectTeleostpor
dc.subjectComparative sequence analysispor
dc.titleOligopeptide transporter PepT1 in Atlantic cod (Gadus morhua L.): cloning, tissue expression and comparative aspectspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3896por
oaire.citation.issue22por
oaire.citation.startPage3883por
oaire.citation.titleJournal of Experimental Biologypor
oaire.citation.volume210por
person.familyNameGavaia
person.familyNameViegas
person.familyNameCancela
person.givenNamePaulo
person.givenNameCarla
person.givenNameM. Leonor
person.identifier.ciencia-idB619-FC16-D007
person.identifier.ciencia-idC414-F596-EEC6
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0002-5765-3665
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridA-6470-2011
person.identifier.ridN-6695-2014
person.identifier.scopus-author-id6507104377
person.identifier.scopus-author-id8656310300
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication9dca2139-21a4-4d59-aaf7-531f1033a58e
relation.isAuthorOfPublication33984b3c-ffac-477a-896a-ddbf7aced4e3
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublication.latestForDiscoveryb9bbfe32-3dfe-4131-ad14-a4394008447f

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