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Immunocytochemistry of somatotrophs, gonadotrophs, prolactin and adrenocorticotropin cells in larval sea bream (Sparus auratus) pituitaries
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Orientador(es)
Resumo(s)
The chronological appearance of endocrine
cells in the pituitary of sea-bream (Sparus auratus) larvae was studied using antisera against salmon prolactin, trout growth hormone, salmon gonadotropin and Nterminal
human adrenocorticotropin. The larval pituitary (1-12 days after hatching) was oval in shape and was composed of a dense mass of cells with few neurohypophysial
fibres. By 60 days after hatching it began to
resemble the adult and was divisible into a distinct rostral pars distalis containing prolactin and adrenocorticotropin cells; a proximal pars distalis containing somatotrophs and gonadotrophs and a pars intermedia. Cells immunoreactive with antisera against growth hormone were observed immediately after hatching (2 days postfertilization). Weakly staining prolactin cells were observed 2 days later in the region corresponding to the
rostral pars distalis. Cells immunoreactive with antigonadotropin and anti-adrenocorticotropin sera were observed in the pituitary 6 and 8 days after hatching,
respectively. All the cell-types studied were immunoreactire from the time they were first identified until the final samples 90 days after hatching.
Descrição
Palavras-chave
Growth hormone Sparus auratus (Teleostei) Pituitary gland Immunocytochemistry Adrenocorticotropin Gonadotropin Prolactin
Contexto Educativo
Citação
Power, D. M.; Canario, A. V. M. Immunocytochemistry of somatotrophs, gonadotrophs, prolactin and adrenocorticotropin cells in larval sea bream (Sparus auratus) pituitaries, Cell & Tissue Research, 269, 2, 341-346, 1992.