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The feasibility of using gas mixture to stun seabream (Sparus aurata) before slaughtering in aquaculture production

2021Journal article Restricted access
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dc.contributor.authorRoque, A.
dc.contributor.authorGras, N.
dc.contributor.authorRey-Planellas, S.
dc.contributor.authorFatsini, Elvira
dc.contributor.authorPallisera, J.
dc.contributor.authorDuncan, N.
dc.contributor.authorMuñoz, I.
dc.contributor.authorVelarde, A.
dc.contributor.authorHernandez, M. D.
dc.date.accessioned2021-10-01T10:27:34Z
dc.date.available2021-10-01T10:27:34Z
dc.date.issued2021
dc.description.abstractCurrent European Union regulation explicitly states that farmed fish should be spared any avoidable pain, distress or suffering at the time of slaughter. It has been shown that fish suffer when they are killed in an ice slurry, the most common method of killing farmed fish in the Mediterranean. Thus, it is necessary to find a method of slaughtering Mediterranean fish that is, (1) efficient in inducing unconsciousness with minimal pain and distress, (2) practical to be applied to a large group of animals at the same time, and (3) feasible to be used at sea. The present study assesses the welfare of Gilthead seabream (Sparus aurata) stunned by two different gas mixtures authorised for stunning other farmed species. To achieve this objective, commercial sized seabream were stunned and/or sacrificed under different protocols: a) killed directly in ice slurry, b) exposed to a mixture of 30% CO2 + 70% N-2, and then moved to ice slurry and c) exposed to a mixture of 40% CO2 + 30% N-2 + 30% O-2 and then moved to ice slurry. Electroencephalograms (EEG) were recorded to evaluate the state of consciousness of seabream during stunning, while blood and brains were sampled to obtain acute stress indicators and relative gene expression, respectively. Additionally, dead fish were kept for in situ meat quality evaluation. When exposed to the gas mixtures, fish lost balance at 1 min 23 s +/- 31 s with CO2 + N-2 and 1 min 12 s +/- 32 s, with CO2 + N-2 + O-2, respectively. Cortisol, lactate and glucose levels were significantly lower in all fish exposed to gas prior to ice slurry than in fish slaughtered directly in ice slurry (p < 0.05). Electroencephalogram records indicated that fish started to lose consciousness when they lost balance and sank to the bottom of the tank. No differences were found in the meat quality (pH and rigor mortis) among the three treatments. Altogether, the study concludes that the use of carbon dioxide together with nitrogen prior to immersion in ice slurry is more humane than ice slurry alone.pt_PT
dc.description.sponsorshipRTA2012–00046–00-00pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.aquaculture.2021.737168pt_PT
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10400.1/17178
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectStunningpt_PT
dc.subjectStress indicatorspt_PT
dc.subjectElectroencephalogrampt_PT
dc.subjectUnconsciousnesspt_PT
dc.subjectSparus auratapt_PT
dc.titleThe feasibility of using gas mixture to stun seabream (Sparus aurata) before slaughtering in aquaculture productionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage737168pt_PT
oaire.citation.titleAquaculturept_PT
oaire.citation.volume545pt_PT
person.familyNameFatsini Fernández
person.givenNameElvira
person.identifierhttps://scholar.google.es/citations?hl=en&pli=1&user=55mbsZIAAAAJ
person.identifier.ciencia-idEA1D-0571-7A6D
person.identifier.orcid0000-0003-0371-5290
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationde251b78-72d6-49ee-bcc2-79db8f64efb8
relation.isAuthorOfPublication.latestForDiscoveryde251b78-72d6-49ee-bcc2-79db8f64efb8

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