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On the Way to the Identification of a Downy Mildew Resistance Gene in Brassica oleracea L.

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dc.contributor.authorCarlier, Jorge
dc.contributor.authorAlabaca, C
dc.contributor.authorRodrigues, C.
dc.contributor.authorLeitão, José
dc.date.accessioned2015-06-19T14:36:30Z
dc.date.available2015-06-19T14:36:30Z
dc.date.issued2013
dc.description.abstractWe have recently mapped the dominant and monogenically inherited downy mildew resistance locus Pp523 to the chromosome C8 of Brassica oleracea L. and constructed a physical-map of the genomic region of this locus. Presently, we are constructing a BAC genomic library of a downy mildew resistant S4 line derived from the broccoli accession originally found to harbor the Pp523 locus and using STS markers derived from the previously constructed physical map in order to identify a contig of BAC clones which sequencing could allow the isolation and cloning of this resistance locus. The new BAC library contains 17280 clones with an average insert size of 125 Kbp, which represent approximately 3.5 equivalents of the broccoli genome. To date, 18 clones from this new library were selected and assembled in a contig (with two small gaps) that covers the major part of the previously physically mapped region. The overlapping between BAC clones has been identified by PCR and confirmed by sequencing of the PCR products. The precise positioning of BAC clones in the genome has been made possible through the alignment of the BAC-end sequences with the genomic sequences of B. oleracea available at the Brassica database (BRAD).
dc.identifier.isbn978-90-66056-46-6
dc.identifier.issn0567-7572
dc.identifier.otherAUT: JLE00446;
dc.identifier.urihttp://hdl.handle.net/10400.1/6538
dc.language.isoeng
dc.peerreviewedyes
dc.publisherInt. Soc Horticultural Science
dc.relationCloning Pp523, a downy mildew resistance gene of Brassica oleracea L.
dc.relationISOLAMENTO DE PP523 E PPALG1, DOIS GENES DOMINANTES DE RESISTÊNCIA AO MÍLDIO EM PLANTAS ADULTAS DE BRASSICA OLERACEA L.
dc.relation.isbasedonP-009-84A
dc.relation.ispartofActa Horticulturae
dc.titleOn the Way to the Identification of a Downy Mildew Resistance Gene in Brassica oleracea L.
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleCloning Pp523, a downy mildew resistance gene of Brassica oleracea L.
oaire.awardTitleISOLAMENTO DE PP523 E PPALG1, DOIS GENES DOMINANTES DE RESISTÊNCIA AO MÍLDIO EM PLANTAS ADULTAS DE BRASSICA OLERACEA L.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-GPL%2F70135%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/PIDDAC/SFRH%2FBPD%2F41714%2F2007/PT
oaire.citation.conferencePlaceCatania, Italy
oaire.citation.endPage237
oaire.citation.startPage233
oaire.citation.titleVI International Symposium on Brassicas and XVIII Crucifer Genetics Workshop
oaire.citation.title6th International Symposium on Brassicas / 18th Crucifer Genetics Workshop
oaire.citation.volume1005
oaire.fundingStream3599-PPCDT
oaire.fundingStreamPIDDAC
person.familyNameCarlier
person.familyNameLeitão
person.givenNameJorge
person.givenNameJosé
person.identifier258398
person.identifier.ciencia-id691D-A1BB-15CC
person.identifier.ciencia-id7A17-3E0A-73AD
person.identifier.orcid0000-0003-0675-2716
person.identifier.orcid0000-0003-4278-0415
person.identifier.ridAAH-3958-2020
person.identifier.scopus-author-id7102166949
person.identifier.scopus-author-id7005119796
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typeconferenceObject
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