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Optimization of sterlet sperm concentration for cryopreservation

2021Journal article Restricted access
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dc.contributor.authorNascimento, João Pedro
dc.contributor.authorHorokhovatskyi, Yevhen
dc.contributor.authorKholodnyy, Vitaliy
dc.contributor.authorRodina, Marek
dc.contributor.authorDzyuba, Viktoriya
dc.contributor.authorStechkina, Taisiya
dc.contributor.authorBoryshpolets, Sergii
dc.contributor.authorDzyuba, Borys
dc.date.accessioned2021-06-22T08:33:58Z
dc.date.available2021-06-22T08:33:58Z
dc.date.issued2021
dc.description.abstractOne of the critical points in low temperature storage is the expense of storage capacities. Moreover, sperm concentration in a sample is not only a parameter for effective use of a cryobanks but also a parameter affecting cryopreservation outcomes. Sturgeon sperm in comparison to other species is significantly less concentrated due to the specificity of sperm maturation process, during which sperm is mixed with urine. In this study, we evaluated whether the artificial modification of the sterlet spermatozoa concentration before freezing can be useful during the routine application of sturgeon sperm cryopreservation. The sperm was first concentrated by centrifugation of native sperm samples. Then, the excess of seminal fluid was collected. The concentrated sperm samples were further diluted with the same seminal fluid to obtain different sperm concentrations in suspension, and they were subjected to freeze-thawing. The percentage of postthaw sperm motility depended on the sperm concentration in the samples. The highest post-thaw sperm motility was found in sperm samples with concentration of 0.2 and 1 × 109 spz mL􀀀 1. The sperm concentration of 3 × 109 spz mL􀀀 1, which is higher than the native sperm concentration in sterlet, was found to be appropriate for use in cryopreservation procedures since the sperm fertilizing ability remained at a high level even though a significant decline in percentage of sperm motility was observed. These findings supported the conclusion that the procedure of artificially increasing the sperm concentration before freezing could be useful for the reduction of the volume kept in cryostorage and to decrease the sample volume required for artificial fertilization.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.aquaculture.2021.736682pt_PT
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10400.1/16333
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationAQUAculture infrastructures for EXCELlence in European fish research towards 2020
dc.subjectSperm motilitypt_PT
dc.subjectSperm concentrationpt_PT
dc.subjectDevelopment and hatching ratept_PT
dc.subjectAcipenser ruthenuspt_PT
dc.subjectSperm velocitypt_PT
dc.titleOptimization of sterlet sperm concentration for cryopreservationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAQUAculture infrastructures for EXCELlence in European fish research towards 2020
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/652831/EU
oaire.citation.startPage736682pt_PT
oaire.citation.titleAquaculturept_PT
oaire.citation.volume540pt_PT
oaire.fundingStreamH2020
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationaf2e9e71-3f77-4149-8f42-1d186fc443e4
relation.isProjectOfPublication.latestForDiscoveryaf2e9e71-3f77-4149-8f42-1d186fc443e4

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