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Abstract(s)
Rocket is the common designation for two baby-leaf salad crops of the Brassicaceae family:
Eruca sativa (L.) Cav., usually referred to as annual garden rocket, and Diplotaxis tenuifolia (L.) DC.
commonly named to as perennial wild rocket. E. sativa is used for human consumption since antiquity.
However, the growing consumer preference for D. tenuifolia is being accompanied by the fast increase
in its production area and commercialization of new cultivars. Nevertheless, the worldwide number
of wild rocket accessions maintained in germplasm collections is very reduced, the solution for which
situation the project “REMIRucula” intends to contribute, establishing a germplasm collection at
the INIAV, Oeiras, Portugal. Herein, we report on the establishment via next generation sequencing
(NGS) of the first genome assembly of D. tenuifolia and the identification of specific single sequence
repeat (SSR) and single nucleotide polymorphisms (SNP) loci for the establishment of specific
DNA-markers for this species. A representative set of 87 D. tenuifolia and 3 E. sativa accessions
were assessed by 5 SSR and 9 SNP-CAPS markers, allowing a drastic discrimination between both
species and the establishment of unequivocal molecular fingerprints for the analyzed accessions. The
non-discrimination within six pairs and one trio of D. tenuifolia accessions is discussed.
Description
Keywords
Diplotaxis tenuifolia Eruca sativa Wild rocket Garden rocket Germplasm collections SSR markers SNP-CAPS markers
Citation
Plants 11 (24): 3482 (2022)
Publisher
MDPI
CC License
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