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Assessment of Wild Rocket (Diplotaxis tenuifolia (L.) DC.) Germplasm accessions by NGS identified SSR and SNP markers

2022-12-12Journal article Open Access
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dc.contributor.authorReis, João M.
dc.contributor.authorPereira, Ricardo
dc.contributor.authorCoelho, Paula S.
dc.contributor.authorLeitão, José
dc.date.accessioned2023-01-06T10:16:48Z
dc.date.available2023-01-06T10:16:48Z
dc.date.issued2022-12-12
dc.date.updated2022-12-22T14:36:04Z
dc.description.abstractRocket is the common designation for two baby-leaf salad crops of the Brassicaceae family: Eruca sativa (L.) Cav., usually referred to as annual garden rocket, and Diplotaxis tenuifolia (L.) DC. commonly named to as perennial wild rocket. E. sativa is used for human consumption since antiquity. However, the growing consumer preference for D. tenuifolia is being accompanied by the fast increase in its production area and commercialization of new cultivars. Nevertheless, the worldwide number of wild rocket accessions maintained in germplasm collections is very reduced, the solution for which situation the project “REMIRucula” intends to contribute, establishing a germplasm collection at the INIAV, Oeiras, Portugal. Herein, we report on the establishment via next generation sequencing (NGS) of the first genome assembly of D. tenuifolia and the identification of specific single sequence repeat (SSR) and single nucleotide polymorphisms (SNP) loci for the establishment of specific DNA-markers for this species. A representative set of 87 D. tenuifolia and 3 E. sativa accessions were assessed by 5 SSR and 9 SNP-CAPS markers, allowing a drastic discrimination between both species and the establishment of unequivocal molecular fingerprints for the analyzed accessions. The non-discrimination within six pairs and one trio of D. tenuifolia accessions is discussed.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPlants 11 (24): 3482 (2022)pt_PT
dc.identifier.doi10.3390/plants11243482pt_PT
dc.identifier.eissn2223-7747
dc.identifier.urihttp://hdl.handle.net/10400.1/18740
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDiplotaxis tenuifoliapt_PT
dc.subjectEruca sativapt_PT
dc.subjectWild rocketpt_PT
dc.subjectGarden rocketpt_PT
dc.subjectGermplasm collectionspt_PT
dc.subjectSSR markerspt_PT
dc.subjectSNP-CAPS markerspt_PT
dc.titleAssessment of Wild Rocket (Diplotaxis tenuifolia (L.) DC.) Germplasm accessions by NGS identified SSR and SNP markerspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FASP-PLA%2F28963%2F2017/PT
oaire.citation.issue24pt_PT
oaire.citation.startPage3482pt_PT
oaire.citation.titlePlantspt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream9471 - RIDTI
person.familyNameReis
person.familyNamePereira
person.familyNameLeitão
person.givenNameJoão
person.givenNameRicardo
person.givenNameJosé
person.identifier258398
person.identifier.ciencia-idC818-CA64-011F
person.identifier.ciencia-id6219-C33A-5BA2
person.identifier.ciencia-id7A17-3E0A-73AD
person.identifier.orcid0000-0002-3461-8904
person.identifier.orcid0000-0002-7358-6300
person.identifier.orcid0000-0003-4278-0415
person.identifier.ridAAH-3958-2020
person.identifier.scopus-author-id7005119796
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication8b194de2-d1fa-438c-bff1-b488dc7b00e1
relation.isAuthorOfPublicationb519d2c4-8c76-42d6-a638-318e8b1ab369
relation.isAuthorOfPublication0f990d1c-2913-4f8a-8fb6-f8b02721247f
relation.isAuthorOfPublication.latestForDiscovery0f990d1c-2913-4f8a-8fb6-f8b02721247f
relation.isProjectOfPublication9fbcb5fe-13fe-491f-8d8a-88c7426cacc0
relation.isProjectOfPublication.latestForDiscovery9fbcb5fe-13fe-491f-8d8a-88c7426cacc0

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