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Interrelationship between contractility, protein synthesis and metabolism in mantle of juvenile cuttlefish (Sepia officinalis)

2019Journal article Open access
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dc.contributor.authorLamarre, Simon G.
dc.contributor.authorMacCormack, Tyson J.
dc.contributor.authorBourloutski, Émilie
dc.contributor.authorCallaghan, Neal I.
dc.contributor.authorPinto, Vanessa
dc.contributor.authorAndrade, José Pedro
dc.contributor.authorSykes, António
dc.contributor.authorDriedzic, William R.
dc.date.accessioned2019-09-23T12:55:38Z
dc.date.available2019-09-23T12:55:38Z
dc.date.issued2019
dc.description.abstractYoung juvenile cuttlefish (Sepia officinalis) can grow at rates as high as 12% body weight per day. How the metabolic demands of such a massive growth rate impacts muscle performance that competes for ATP is unknown. Here, we integrate aspects of contractility, protein synthesis, and energy metabolism in mantle of specimens weighing 1.1 g to lend insight into the processes. Isolated mantle muscle preparations were electrically stimulated and isometric force development monitored. Preparations were forced to contract at 3 Hz for 30 s to simulate a jetting event. We then measured oxygen consumption, glucose uptake and protein synthesis in the hour following the stimulation. Protein synthesis was inhibited with cycloheximide and glycolysis was inhibited with iodoacetic acid in a subset of samples. Inhibition of protein synthesis impaired contractility and decreased oxygen consumption. An intact protein synthesis is required to maintain contractility possibly due to rapidly turning over proteins. At least, 41% of whole animal ṀO2 is used to support protein synthesis in mantle, while the cost of protein synthesis (50 μmol O2 mg protein-1) in mantle was in the range reported for other aquatic ectotherms. A single jetting challenge stimulated protein synthesis by approximately 25% (2.51-3.12% day-1) over a 1 h post contractile period, a similar response to that which occurs in mammalian skeletal muscle. Aerobic metabolism was not supported by extracellular glucose leading to the contention that at this life stage either glycogen or amino acids are catabolized. Regardless, an intact glycolysis is required to support contractile performance and protein synthesis in resting muscle. It is proposed that glycolysis is needed to maintain intracellular ionic gradients. Intracellular glucose at approximately 3 mmol L-1 was higher than the 1 mmol L-1 glucose in the bathing medium suggesting an active glucose transport mechanism. Octopine did not accumulate during a single physiologically relevant jetting challenge; however, octopine accumulation increased following a stress that is sufficient to lower Arg-P and increase free arginine.pt_PT
dc.description.sponsorshipAgência financiadora Natural Sciences and Engineering Research Council of Canada (NSERC) NSERC CPB section of the Canadian Society of Zoologists Fundacao para a Ciencia e a Tecnologia (FCT) IF/00576/2014 Portuguese national funds from Programa Operacional Mar2020 (Portugal2020/FEAMP) - Project SEPIACUL 16-02-01-FMP-53 FCT through Plurennial UID/Multi/04326/2019 EMBRC. PT ALG-01-0145-FEDER-022121 Portuguese node of EMBRC-ERICpt_PT
dc.description.sponsorshipALG-01-0145-FEDER-022121
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3389/fphys.2019.01051
dc.identifier.issn1664-042X
dc.identifier.urihttp://hdl.handle.net/10400.1/12773
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Mediapt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnaerobic metabolismpt_PT
dc.subjectCycloheximidept_PT
dc.subjectGlucosept_PT
dc.subjectIodoacetic acidpt_PT
dc.subjectJettingpt_PT
dc.subjectOctopinept_PT
dc.titleInterrelationship between contractility, protein synthesis and metabolism in mantle of juvenile cuttlefish (Sepia officinalis)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage1051pt_PT
oaire.citation.titleFrontiers in Physiologypt_PT
oaire.citation.volume10pt_PT
person.familyNameAndrade
person.familyNameSykes
person.givenNameJose
person.givenNameAntónio
person.identifier107454
person.identifier.ciencia-idD514-1A21-F020
person.identifier.ciencia-id7510-6641-5A42
person.identifier.orcid0000-0002-5482-9776
person.identifier.orcid0000-0002-5207-0612
person.identifier.ridC-3609-2012
person.identifier.scopus-author-id7201722097
person.identifier.scopus-author-id7102626426
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication8f1ffbdc-85aa-4342-8bd8-61eb6d6be74e
relation.isAuthorOfPublicationb9d55f95-5de8-40cf-82a3-96ec98c91a55
relation.isAuthorOfPublication.latestForDiscovery8f1ffbdc-85aa-4342-8bd8-61eb6d6be74e

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