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Olfactory transduction pathways in the Senegalese sole Solea senegalensis

2013Journal article Restricted access
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dc.contributor.authorVelez, Zélia
dc.contributor.authorHubbard, Peter
dc.contributor.authorBarata, E. N.
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-05-12T10:14:11Z
dc.date.available2014-05-12T10:14:11Z
dc.date.issued2013
dc.date.updated2014-05-08T09:35:57Z
dc.description.abstractThis study tested whether differences in sensitivity between the upper and lower olfactory epithelia of Solea senegalensis are associated with different odorant receptors and transduction pathways, using the electro-olfactogram. Receptor mechanisms were assessed by cross-adaptation with amino acids (L-cysteine, L-phenylalanine and 1-methyl-L-tryptophan) and bile acids (taurocholic acid and cholic acid). This suggested that relatively specific receptors exist for 1-methyl-L-tryptophan and Lphenylalanine (food-related odorants) in the lower epithelium, and for taurocholic acid (conspecificderived odorant) in the upper. Inhibition by U73122 [a phospholipase C (PLC) inhibitor] suggested that olfactory responses to amino acids were mediated mostly, but not entirely, by PLC-mediated transduction (IC50; 15–55 nM), whereas bile acid responses were mediated by both PLC and adenylate cyclase–cyclic adenosine monophosphate (AC–cAMP) (using SQ-22536; an AC inhibitor). Simultaneous application of both drugs rarely inhibited responses completely, suggesting possible involvement of non-PLC and non-AC mediated mechanisms. For aromatic amino acids and bile acids, there were differences in the contribution of each transduction pathway (PLC, AC and non-PLC and non-AC) between the two epithelia. These results suggest that differences in sensitivity of the two epithelia are associated with differences in odorant receptors and transduction mechanisms.por
dc.identifier.citationVelez, Z.; Hubbard, P. C.; Barata, E. N.; Canário, A. V. M. Olfactory transduction pathways in the Senegalese sole Solea senegalensis , Journal of Fish Biology, 83, 3, 501-514, 2013.por
dc.identifier.doihttp://dx.doi.org/10.1111/jfb.12185
dc.identifier.issn0022-1112
dc.identifier.otherAUT: ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3858
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.subjectAdenylate cyclasepor
dc.subjectPhospholipase Cpor
dc.subjectElectro-olfactogrampor
dc.subjectBile acidpor
dc.subjectAsymmetrypor
dc.subjectAmino acidpor
dc.titleOlfactory transduction pathways in the Senegalese sole Solea senegalensispor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage504por
oaire.citation.issue3por
oaire.citation.startPage501por
oaire.citation.titleJournal of Fish Biologypor
oaire.citation.volume83por
person.familyNameVelez
person.familyNameHubbard
person.familyNameBarata
person.familyNameCanario
person.givenNameZélia
person.givenNamePeter
person.givenNameEduardo Nuno
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-id4412-F95B-34A2
person.identifier.ciencia-id951F-BA4B-A21F
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0003-2761-4048
person.identifier.orcid0000-0002-3007-4647
person.identifier.orcid0000-0001-9714-5967
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridM-5283-2013
person.identifier.ridB-2823-2008
person.identifier.ridB-2902-2010
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id16176444400
person.identifier.scopus-author-id7005928467
person.identifier.scopus-author-id6602073256
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery3292b0fd-8d08-4512-b512-a04b31a7926a

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