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Roots and rhizomes of wild Asparagus: nutritional composition, bioactivity and nanoencapsulation of the most potent extract

2022Journal article Restricted access
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dc.contributor.authorAdouni, Khaoula
dc.contributor.authorJúlio, Ana
dc.contributor.authorSantos-Buelga, Celestino
dc.contributor.authorGonzález-Paramás, Ana M.
dc.contributor.authorFilipe, Patrícia
dc.contributor.authorRijo, Patricia
dc.contributor.authorCosta Lima, Sofia A.
dc.contributor.authorReis, Salette
dc.contributor.authorFernandes, Ângela
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorFernández-Ruiz, Virginia
dc.contributor.authorMorales, Patricia
dc.contributor.authorFlamini, Guido
dc.contributor.authorAchour, Lotfi
dc.contributor.authorFonte, Pedro
dc.date.accessioned2022-07-28T13:08:46Z
dc.date.available2022-08-24T00:30:16Z
dc.date.issued2022
dc.description.abstractThe nutritional composition and bioactive properties of roots and rhizomes of Asparagus stipularis were evalu- ated. Antioxidant activity of extracts obtained by infusion was evaluated using free radicals scavenging and reducing power methods. Porcine liver primary cell was used to check the hepatotoxicity of infusions. Results revealed that Asparagus samples are likely a source of nutrients, such as dietary fibre and essential fatty acids. HPLC-DAD-ESI/MS characterization of infusions allowed the identification and quantitation of 7 phenolic compounds, all hydroxycinnamoyl derivatives, with caffeic acid as the most abundant. Roots infusion contained the highest amounts of these compounds. It also exhibited the highest antioxidant activity in all assays, with EC50 values of 0.44 ± 0.01, 0.98 ± 0.03 and 0.64 ± 0.01 mg/mL for DPPH, ABTS and FRAP assays, respectively, with no toxicity towards PLP2 primary cell cultures (GI50 > 400 μg/mL). PLGA nanoparticles loaded with root extract were prepared using solvent-evaporation double emulsion method. Nanoparticles size was about 260 nm and a polydispersity index around 0.1, with a zeta potential of about -36 mV, as well as a good encapsulation efficiency of approximately 83%. Their morphology was analysed by SEM and spherical polymeric nanoparticles with a smooth surface were observed. FTIR and DSC were also performed, which allowed corroborating the efficacy of the encapsulation and to confirm the production of a stable and robust system to load Asparagus extracts. The developed nanoparticles are expected to be used as delivery systems for bioactive compounds of A. stipularis and they could be used as an innovative dietary supplement.pt_PT
dc.description.sponsorshipAGL201564522-C2-2-R; PID2019-109365RA-I00; UCM GR105/18
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.fbio.2021.101334pt_PT
dc.identifier.issn2212-4292
dc.identifier.urihttp://hdl.handle.net/10400.1/18127
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationMountain Research Center
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationAlgarve Centre for Marine Sciences
dc.relationResearch Center for Biosciences & Health Technologies
dc.relationInstitute for Bioengineering and Biosciences
dc.subjectAsparagus stipularispt_PT
dc.subjectNutritional compositionpt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectNanoparticlespt_PT
dc.subjectPLGApt_PT
dc.titleRoots and rhizomes of wild Asparagus: nutritional composition, bioactivity and nanoencapsulation of the most potent extractpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleResearch Center for Biosciences & Health Technologies
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04567%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04565%2F2020/PT
oaire.citation.startPage101334pt_PT
oaire.citation.titleFood Biosciencept_PT
oaire.citation.volume45pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFonte
person.givenNamePedro
person.identifier.ciencia-id2410-123D-3385
person.identifier.orcid0000-0002-1115-9282
person.identifier.ridK-3215-2013
person.identifier.scopus-author-id55146900200
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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