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Effects of the hydrodynamic environment and oxygen mass transfer on plant cell growth and milk-clotting protease production in a stirred-tank reactor

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dc.contributor.authorRaposo, Sara
dc.contributor.authorLima-Costa, Maria Emília
dc.date.accessioned2014-07-23T10:28:33Z
dc.date.available2014-07-23T10:28:33Z
dc.date.issued2012
dc.date.updated2014-07-22T10:07:03Z
dc.description.abstractIndustrial-scale plant cell based bioprocesses are limited because of technological challenges associated with mass transfer and mixing in the heterogeneous broth, which typically displays non-Newtonian characteristics. Cultures of Centaurea calcitrapa cells, presenting milk-clotting activity, were established in a 7-L stirred-tank reactor (STR), keeping the initial mass transfer conditions constant, to gain a better understanding of the effects of the hydrodynamic environment and mass transfer on protease production. Cell suspensions grown in an STR equipped with one single marine propeller or two Rushton turbines showed the highest protease activities and biomass production, corresponding to the adequate mass transfer achieved under the tested bioreactor conditions. The key factors improving milk-clotting protease production in a bioreactor were an efficient mass transfer and good bulk mixing without the formation of stagnant zones, while a compromise had to be established in relation to the hydrodynamic shear conditions.por
dc.identifier.citationRaposo, Sara; Lima-Costa, Maria Emília. Effects of the hydrodynamic environment and oxygen mass transfer on plant cell growth and milk-clotting protease production in a stirred-tank reactor, Engineering in Life Sciences, 12, 4, 441-449, 2012.por
dc.identifier.doihttp://dx.doi.org/
dc.identifier.doi10.1002/elsc.201100087
dc.identifier.issn16180240
dc.identifier.otherAUT: SRA01180;
dc.identifier.urihttp://hdl.handle.net/10400.1/4805
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/elsc.201100087/abstractpor
dc.subjectMixingpor
dc.subjectPlant cell culturepor
dc.subjectProteolytic activitypor
dc.subjectShear stresspor
dc.subjectSTRpor
dc.titleEffects of the hydrodynamic environment and oxygen mass transfer on plant cell growth and milk-clotting protease production in a stirred-tank reactorpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage449por
oaire.citation.issue4por
oaire.citation.startPage441por
oaire.citation.titleEngineering in Life Sciencespor
oaire.citation.volume12por
person.familyNameRaposo
person.familyNameLima Costa
person.givenNameSara
person.givenNameMaria Emília
person.identifier.ciencia-idF010-54F2-9E30
person.identifier.orcid0000-0001-5344-7349
person.identifier.orcid0000-0002-4433-5756
person.identifier.ridL-3673-2013
person.identifier.scopus-author-id7801639810
person.identifier.scopus-author-id7003633118
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication035b3010-5f47-4700-b196-402970ff2a69
relation.isAuthorOfPublication6eb9b8a6-dd51-4ace-809b-7d7de9cdfe36
relation.isAuthorOfPublication.latestForDiscovery035b3010-5f47-4700-b196-402970ff2a69

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