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Solea senegalensis vasa transcripts: molecular characterisation, tissue distribution and developmental expression profiles

2013Journal article Restricted access
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dc.contributor.authorPacchiarini, T.
dc.contributor.authorCross, I.
dc.contributor.authorLeite, Ricardo
dc.contributor.authorGavaia, Paulo J.
dc.contributor.authorOrtiz-Delgado, J. B.
dc.contributor.authorPousão-Ferreira, P.
dc.contributor.authorRebordinos, L.
dc.contributor.authorSarasquete, C.
dc.contributor.authorCabrita, Elsa
dc.date.accessioned2014-06-05T13:46:32Z
dc.date.available2014-06-05T13:46:32Z
dc.date.issued2013
dc.date.updated2014-05-30T09:57:41Z
dc.description.abstractThe Vasa protein is an RNA helicase belonging the DEAD (Asp-Glu-Ala-Asp)-box family. The crucial role played by the vasa gene in the germ-cell lineage of both vertebrates and invertebrates has made this gene a useful molecular marker for germinal cells and a useful tool in surrogate broodstock production using primordial germ cell transplantation. With the aim of establishing a novel approach to improving Solea senegalensis broodstock management, the vasa gene in this species was characterised. Four S. senegalensis vasa transcripts were isolated: Ssvasa1, Ssvasa2, Ssvasa3 and Ssvasa4. Their phylogenetic relationship with other vasa homologues was determined confirming the high degree of conservation of this helicase throughout evolution. Our qPCR results showed that S. senegalensis vasa transcripts are prevalently expressed in gonads, with ovary-specific expression for Ssvasa3 and Ssvasa4. During embryonic and larval development, a switch between the longest and the shortest transcripts was observed. While Ssvasa1 and Ssvasa2 were maternally supplied, Ssvasa3 and Ssvasa4 depended on the de novo expression program of the growing juveniles, suggesting that vasa mRNA could be involved in Senegalese sole gonad differentiation. In situ hybridisation and immunohistochemical analysis performed in 150-days after hatching (DAH) larvae showed vasa product expression in the germinal region of early gonads. In our work we demonstrated the usefulness of Ssvasa mRNAs as molecular markers for primordial germ cells and germinal cells during embryonic development, larval ontogenesis and gonad differentiation. Furthermore, our results confirmed the potential of vasa to help investigate germinal cell biotechnology for Senegalese sole reproduction.por
dc.identifier.citationPacchiarini, T.; Cross, I.; Leite, R.B.; Gavaia, P.J.; Ortiz-Delgado, J.B.; Pousão-Ferreira, P.; Rebordinos, L.; Sarasquete, C.; Cabrita, E. Solea senegalensis vasa transcripts: Molecular characterisation, tissue distribution and developmental expression profiles, Reproduction, Fertility and Development, 25, 4, 646-660, 2013.por
dc.identifier.doihttp://dx.doi.org/10.1071/RD11240
dc.identifier.issn1031-3613
dc.identifier.urihttp://hdl.handle.net/10400.1/4201
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherCSIRO Publishingpor
dc.subjectDevelopmentpor
dc.subjectOvarypor
dc.subjectPrimordial germ cellspor
dc.subjectTestispor
dc.subjectVasa expressionpor
dc.titleSolea senegalensis vasa transcripts: molecular characterisation, tissue distribution and developmental expression profilespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage660por
oaire.citation.issue4por
oaire.citation.startPage646por
oaire.citation.titleReproduction, Fertility and Developmentpor
oaire.citation.volume25por
person.familyNameLeite
person.familyNameGavaia
person.familyNamePousão-Ferreira
person.familyNameCabrita
person.givenNameRicardo
person.givenNamePaulo
person.givenNamePedro
person.givenNameElsa
person.identifier447622
person.identifier.ciencia-idF115-256E-C84D
person.identifier.ciencia-idB619-FC16-D007
person.identifier.ciencia-id5A11-AB29-0849
person.identifier.orcid0000-0002-9622-3895
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0001-6746-764X
person.identifier.orcid0000-0003-3864-8841
person.identifier.ridB-3389-2008
person.identifier.ridA-6470-2011
person.identifier.ridH-3689-2014
person.identifier.ridE-8523-2012
person.identifier.scopus-author-id8362650900
person.identifier.scopus-author-id6507104377
person.identifier.scopus-author-id6506349877
person.identifier.scopus-author-id26645697000
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication.latestForDiscovery9dca2139-21a4-4d59-aaf7-531f1033a58e

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