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Human oocyte meiotic maturation is associated with a specific profile of alternatively spliced transcript isoforms

2021Journal article Restricted access
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dc.contributor.authorCornet‐Bartolomé, David
dc.contributor.authorBarragán, Montserrat
dc.contributor.authorZambelli, Filippo
dc.contributor.authorFerrer‐Vaquer, Anna
dc.contributor.authorTiscornia, Gustavo
dc.contributor.authorBalcells, Susanna
dc.contributor.authorRodriguez, Amelia
dc.contributor.authorGrinberg, Daniel
dc.contributor.authorVassena, Rita
dc.date.accessioned2021-09-27T16:07:32Z
dc.date.available2021-09-27T16:07:32Z
dc.date.issued2021
dc.description.abstractThe transition from a transcriptionally active state (GV) to a transcriptionally inactive state (mature MII oocytes) is required for the acquisition of oocyte developmental competence. We hypothesize that the expression of specific genes at the in vivo matured (MII) stage could be modulated by posttranscriptional mechanisms, particularly regulation of alternative splicing (AS). In this study, we examined the transcriptional activity of GV oocytes after ovarian stimulation followed by oocyte pick-up and the landscape of alternatively spliced isoforms in human MII oocytes. Individual oocytes were processed and analyzed for transcriptional activity (GV), gene expression (GV and MII), and AS signatures (GV and MII) on HTA 2.0 microarrays. Samples were grouped according to maturation stage, and then subgrouped according to women's age and antral follicular count (AFC); array results were validated by quantitative polymerase chain reaction. Differentially expressed genes between GV and MII oocytes clustered mainly in biological processes related to mitochondrial metabolism. Interestingly, 16 genes that were related to the regulation of transcription and mitochondrial translation showed differences in alternatively spliced isoform profiles despite not being differentially expressed between groups. Altogether, our results contribute to our understanding of the role of AS in oocyte developmental competence acquisition.pt_PT
dc.description.sponsorshipSecretary for Universities and Research of the Ministry of Economy and Knowledge of the Government of Catalonia GENCAT 2015 DI 048.pt_PT
dc.description.sponsorshipGENCAT 2015 DI 048
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1002/mrd.23526pt_PT
dc.identifier.issn1040-452X
dc.identifier.urihttp://hdl.handle.net/10400.1/17164
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.subjectAlternative splicingpt_PT
dc.subjectAntral follicular countpt_PT
dc.subjectDevelopmental competencept_PT
dc.subjectHuman oocytept_PT
dc.titleHuman oocyte meiotic maturation is associated with a specific profile of alternatively spliced transcript isoformspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage617pt_PT
oaire.citation.issue9pt_PT
oaire.citation.startPage605pt_PT
oaire.citation.titleMolecular Reproduction and Developmentpt_PT
oaire.citation.volume88pt_PT
person.familyNameTiscornia
person.givenNameGustavo
person.identifier.ciencia-id7818-59E3-E4EE
person.identifier.orcid0000-0002-1841-5330
person.identifier.scopus-author-id6507863404
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication8d2c4950-f020-4c0c-bbbd-52d19e1397a4
relation.isAuthorOfPublication.latestForDiscovery8d2c4950-f020-4c0c-bbbd-52d19e1397a4

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