Publication
In vitro minimum growth for conservation of Drosophyllum lusitanicum
| dc.contributor.author | Gonçalves, Sandra | |
| dc.contributor.author | Romano, Anabela | |
| dc.date.accessioned | 2013-03-13T11:49:15Z | |
| dc.date.available | 2013-03-13T11:49:15Z | |
| dc.date.issued | 2007 | |
| dc.description.abstract | The present paper reports a protocol for minimum growth conservation of Drosophyllum lusitanicum (L.) Link. in vitro. Double-node cuttings were maintained for 4, 8 and 12 months at 5 or 25 ºC in the dark. The effects of sucrose either alone at 5, 20, 30, 40 and 60 g dm-3 or at 20, 40 and 60 g dm-3 in combination with 20 g dm-3 mannitol, on survival and post-storage shoot multiplication efficiency were investigated. The cultures could effectively be conserved under minimum growth at 5 ºC for 8 months on Murashige and Skoog’s medium supplemented with 60 g dm-3 sucrose, 20 g dm-3 mannitol and 0.91 μM zeatin. Following extended conservation, the cultures could be successfully regenerated into new shoots, and they were morphologically similar to those of non-stored controls. | por |
| dc.identifier.citation | Gonçalves S., Romano A. 2007. In vitro minimal growth conservation of Drosophyllum lusitanicum (L.) Link, Biologia Plantarum 51: 795-798 | por |
| dc.identifier.issn | 0006-3134 | |
| dc.identifier.other | AUT: ARO01015; | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/2557 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.relation.publisherversion | http://link.springer.com/article/10.1007%2Fs10535-007-0163-0?LI=true | por |
| dc.subject | Endangered species | por |
| dc.subject | Germplasm conservation | por |
| dc.subject | Osmotic stress | por |
| dc.title | In vitro minimum growth for conservation of Drosophyllum lusitanicum | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 798 | por |
| oaire.citation.issue | 4 | por |
| oaire.citation.startPage | 795 | por |
| oaire.citation.title | Biologia Plantarum | por |
| oaire.citation.volume | 51 | por |
| person.familyName | Gonçalves | |
| person.familyName | Romano | |
| person.givenName | Sandra | |
| person.givenName | Anabela | |
| person.identifier.ciencia-id | 9F19-E3AE-2C59 | |
| person.identifier.ciencia-id | 8A18-7E9A-3B0E | |
| person.identifier.orcid | 0000-0002-3038-4434 | |
| person.identifier.orcid | 0000-0002-7204-7428 | |
| person.identifier.rid | M-6988-2017 | |
| person.identifier.scopus-author-id | 8583003200 | |
| person.identifier.scopus-author-id | 56249997600 | |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | a8b335b2-45cc-4578-89b0-153273014280 | |
| relation.isAuthorOfPublication | e3343b60-17d3-4f5e-a2bf-06f26da69206 | |
| relation.isAuthorOfPublication.latestForDiscovery | a8b335b2-45cc-4578-89b0-153273014280 |