Repository logo
 
Publication

Dietary methionine supplementation improves the European seabass (Dicentrarchus labrax) immune status following long-term feeding on fishmeal-free diets

2020-11Journal article Restricted access
Simple item page
dc.contributor.authorMachado, Marina
dc.contributor.authorEngrola, Sofia
dc.contributor.authorColen, R.
dc.contributor.authorConceicao, Luis E. C.
dc.contributor.authorDias, Jorge
dc.contributor.authorCostas, Benjamin
dc.date.accessioned2021-06-18T16:25:47Z
dc.date.available2021-06-18T16:25:47Z
dc.date.issued2020-11
dc.description.abstractMethionine is a limiting amino acid (AA) in fish diets, particularly in those containing high levels of plant protein (PP), and is key in the immune system. Accordingly, outcome on the fish immune mechanisms of methionine-deficient and methionine-supplemented diets within the context of 0 % fishmeal formulation, after a short and prolonged feeding period, was studied in European seabass (Dicentrarchus labrax). For this, seabass juveniles were fed a (i) fishmeal-free diet, meeting AA requirements, but deficient in methionine (MET0·65); (ii) as control, the MET0·65 supplemented with L-methionine at 0·22 % of feed weight (CTRL); (iii) two diets, identical to MET0·65 but supplemented at 0·63 and 0·88 % of feed weight of L-methionine (MET1·25 and MET1·5, respectively); and (iv) a fishmeal-based diet (FM), as positive control. After 2 and 12 weeks of feeding, blood and plasma were sampled for leucocyte counting and humoral parameter assays and head-kidney collected for gene expression. After 2 weeks of feeding, a fishmeal-free diet supplemented with methionine led to changes in the expression of methionine- and leucocyte-related genes. A methionine immune-enhancer role was more evident after 12 weeks with an increased neutrophil percentage and a decreased expression of apoptotic genes, possibly indicating an enhancement of fish immunity by methionine dietary supplementation. Furthermore, even though CTRL and FM present similar methionine content, CTRL presented a reduced expression of several immune-related genes indicating that in a practical PP-based diet scenario, the requirement level of methionine for an optimal immune status could be higher.
dc.description.sponsorshipProjects ALISSA - Portugal [ALG-01-0247-FEDER-3520, European Union through FEDER, COMPETE 2020; CRESC Algarve 2020
dc.identifier.doi10.1017/S0007114520001877
dc.identifier.issn0007-1145
dc.identifier.urihttp://hdl.handle.net/10400.1/15672
dc.language.isoeng
dc.peerreviewedyes
dc.publisherCambridge University Press
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.relationCentre of Marine Sciences
dc.relationMediators of inflammation: unravelling the role of methionine and tryptophan during infection
dc.subjectAmino acids
dc.subjectImmunostimulation
dc.subjectFish
dc.subjectPlant protein
dc.subjectFunctional feed
dc.subject.otherNutrition & Dietetics
dc.titleDietary methionine supplementation improves the European seabass (Dicentrarchus labrax) immune status following long-term feeding on fishmeal-free diets
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardTitleCentre of Marine Sciences
oaire.awardTitleMediators of inflammation: unravelling the role of methionine and tryptophan during infection
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00482%2F2014%2FCP1217%2FCT0005/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00197%2F2015%2FCP1277%2FCT0001/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04423%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04326%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F108243%2F2015/PT
oaire.citation.endPage902
oaire.citation.issue9
oaire.citation.titleBritish Journal of Nutrition
oaire.citation.volume124
oaire.fundingStreamInvestigador FCT
oaire.fundingStreamInvestigador FCT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_NORTE
person.familyNameEngrola
person.familyNamePontes Barbosa Colen
person.givenNameSofia
person.givenNameRita Isabel
person.identifier237846
person.identifier.ciencia-idA610-29E7-9B83
person.identifier.ciencia-idF113-B354-F356
person.identifier.orcid0000-0002-5244-5541
person.identifier.orcid0000-0001-7104-1844
person.identifier.ridA-2485-2012
person.identifier.scopus-author-id8669620600
person.identifier.scopus-author-id55323905000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication51be5a20-df9c-4a90-85eb-51d779642caa
relation.isAuthorOfPublication6aa7f225-0c1b-4d34-aede-b0e1ef925083
relation.isAuthorOfPublication.latestForDiscovery6aa7f225-0c1b-4d34-aede-b0e1ef925083
relation.isProjectOfPublication6c62de73-4af8-4561-9a16-138dfe00ba08
relation.isProjectOfPublicationb8c1fbf8-376a-44c2-98c4-5c1a2c966668
relation.isProjectOfPublication6d853b3e-08dd-460b-b96a-f7538c0ba879
relation.isProjectOfPublicationf38dbd9d-3734-4f3c-8d74-dff8dcaa8674
relation.isProjectOfPublication25b0a44a-352b-40e7-909c-53f601eff468
relation.isProjectOfPublication.latestForDiscoveryf38dbd9d-3734-4f3c-8d74-dff8dcaa8674

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
dietary-methionine-supplementation-improves-the-european-seabass-dicentrarchus-labrax-immune-status-following-long-term-feeding-on-fishmeal-free-diets.pdf
Size:
610.66 KB
Format:
Adobe Portable Document Format
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)