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Genetic diversity of two evergreen oaks [Quercus suber (L.) and Quercus ilex subsp rotundifolia (Lam.)] in Portugal using AFLP markers

2006Journal article Open Access
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dc.contributor.authorCoelho, Ana Cristina
dc.contributor.authorLima, M. B.
dc.contributor.authorNeves, D.
dc.contributor.authorCravador, Alfredo
dc.date.accessioned2018-12-07T14:58:38Z
dc.date.available2018-12-07T14:58:38Z
dc.date.issued2006
dc.description.abstractThe genetic variability of cork oak (Quercus suber, L.) in Portugal was evaluated by AFLP using five primer combinations. Three hundred and thirteen trees from three geographically contrasting regions exhibited a high level of genetic variation. The genetic profile of each individual is composed of 291 loci, randomly positioned in the genome and consists of monomorphic and polymorphic fragments. Similarities and dissimilarities among the individuals were quantitatively evaluated by numerical taxonomy. The overall sample shows a proportion of AFLP polymorphic markers of 71%, denoting a high level of variability. Ninety percent of the polymorphic markers identified in cork oak genotypes are uniformly distributed throughout the cork oak populations of Algarve, Alentejo and Tras-os-Montes regions. The coefficients of genetic similarity vary from 0.61 to 0.88 implying that 60% of fragments found are common. A sample of 52 holm oak [Quercus ilex subsp. rotundifolia (Lam.)] trees from overlapping areas was also analysed by AFLP with the same five primer combinations. However the codification of markers together with those selected on cork oak profiles was feasible with only one primer combination due to an apparent much higher polymorphism. AFLP and numerical taxonomy analysis enabled to differentiate the taxa and showed that the level of similarity observed between the profiles of the individuals from holm oak species was lower than that observed in cork oak, implying that apparently the degree of polymorphism is higher in Q. ilex subsp. rotun-difolia than that quantified in Q. suber. A Bayesian approach was used to assess Q. suber total genetic diversity (Ht = 0.2534, P < 0.001) of which 1.7% (Fst = 0.0172, P < 0.001) was assigned to differences among populations. Analysis of molecular variance (AMOVA) showed that most genetic variation is comprised within populations (96%) while 3.6% is among populations (Phi st = 0.036, P < 0.001). Differences among populations within geographic regions account for 2.6% (Phi sc = 0.026, P < 0.001) of the total variation and only 1.3% (Phi ct = 0.013, P = 0.007) is attributed to variation among regions denoting little differentiation of populations over a range of 700 km.
dc.description.sponsorshipSfS PO-CORKOAKS II
dc.identifier.doi10.1515/sg-2006-0016
dc.identifier.issn0037-5349
dc.identifier.urihttp://hdl.handle.net/10400.1/12127
dc.language.isoeng
dc.peerreviewedyes
dc.publisherJD Sauerlaender's Verlag
dc.relationInvolvement of Phytophthora cinnamomi in the decline disease of Quercus suber. Epidemiological and molecular studies.
dc.subjectCork-Oak
dc.subjectPhytophthora-Cinnamomi
dc.subjectPopulation-Structure
dc.subjectRobur L.
dc.subjectDifferentiation
dc.subjectMicrosatellites
dc.subjectPhylogeography
dc.subjectIdentification
dc.subjectIntrogression
dc.subjectHybridization
dc.titleGenetic diversity of two evergreen oaks [Quercus suber (L.) and Quercus ilex subsp rotundifolia (Lam.)] in Portugal using AFLP markers
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInvolvement of Phytophthora cinnamomi in the decline disease of Quercus suber. Epidemiological and molecular studies.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCTI%2FAGR%2F34389%2F2000/PT
oaire.citation.endPage118
oaire.citation.issue3
oaire.citation.startPage105
oaire.citation.titleSilvae Genetica
oaire.citation.volume55
oaire.fundingStreamPOCI
person.familyNameCoelho
person.familyNameCravador
person.givenNameAna Cristina
person.givenNameAlfredo
person.identifier.ciencia-idBE1B-B52D-1576
person.identifier.orcid0000-0002-2919-5468
person.identifier.orcid0000-0002-9831-9815
person.identifier.ridK-7247-2012
person.identifier.scopus-author-id22940342200
person.identifier.scopus-author-id6602537257
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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relation.isAuthorOfPublication8c84a9e6-6b7f-4b50-93a2-152c85901722
relation.isAuthorOfPublication.latestForDiscovery4ff4975d-8e05-4b75-aeb9-f400520a41d6
relation.isProjectOfPublication3dc34ad0-4c81-4fce-897e-e24e7594dda9
relation.isProjectOfPublication.latestForDiscovery3dc34ad0-4c81-4fce-897e-e24e7594dda9

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